Preprints
https://doi.org/10.5194/mr-2021-16
https://doi.org/10.5194/mr-2021-16

  10 Feb 2021

10 Feb 2021

Review status: this preprint is currently under review for the journal MR.

Real-time NMR spectroscopy in the study of biomolecular kinetics and dynamics

György Pintér1, Katharina F. Hohmann1, J. Tassilo Grün1, Julia Wirmer-Bartoschek1, Clemens Glaubitz2, Boris Fürtig1, and Harald Schwalbe1 György Pintér et al.
  • 1Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-Universität Frankfurt, Frankfurt, 60438, Germany
  • 2Institute for Biophysical Chemistry, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-Universität Frankfurt, Frankfurt, 60438, Germany

Abstract. The review describes the application of NMR spectroscopy to study kinetics of folding, refolding and aggregation of proteins, RNA and DNA. Time-resolved NMR experiments can be conducted in a reversible or an irreversible manner. In particular irreversible folding experiments pose large requirements on (i) the signal-to-noise due to the time limitations and (ii) on synchronizing the refolding steps. Thus, this contribution discusses the application of methods for signal-to-noise increases including dynamic nuclear polarization, hyperpolarization and photo-CIDNP for the study of time-resolved NMR studies. Further, methods are reviewed ranging from pressure- and temperature-jump, light induction and rapid mixing to induce rapidly non-equilibrium conditions required to initiate folding.

György Pintér et al.

Status: open (until 21 Mar 2021)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2021-16', Peter Hore, 15 Feb 2021 reply
    • AC1: 'Reply on RC1', Harald Schwalbe, 15 Feb 2021 reply

György Pintér et al.

György Pintér et al.

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Short summary
The folding, refolding and misfolding of biomacromolecules including proteins, DNA and RNA is an important area of biophysical research to understand functional and disease states of a cell. NMR spectroscopy provides detailed insight, both with high time and atomic resolution. These experiments put stringent requirements on signal-to-noise for often irreversible folding reactions. The review describes methodological approaches and highlights key applications.