Preprints
https://doi.org/10.5194/mr-2021-24
https://doi.org/10.5194/mr-2021-24

  05 Mar 2021

05 Mar 2021

Review status: a revised version of this preprint was accepted for the journal MR and is expected to appear here in due course.

Using delayed decoupling to attenuate residual signals in editing filters

Kenneth A. Marincin1, Indrani Pal1,a, and Dominique P. Frueh1 Kenneth A. Marincin et al.
  • 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA
  • acurrent address: Department of Biochemistry and Molecular Biophysics, Kansas State University

Abstract. Isotope filtering methods are instrumental in biomolecular nuclear magnetic resonance (NMR) studies as they isolate signals of chemical moieties of interest within complex molecular assemblies. However, isotope filters suppress undesired signals of isotopically enriched molecules through scalar couplings, and variations in scalar couplings lead to imperfect suppressions, as occurs for aliphatic and aromatic moieties in proteins. Here, we show that signals that have escaped traditional filters can be attenuated without sensitivity losses for the desired signals of unlabeled moieties. The method uses a shared evolution between the detection and preceding preparation period to establish non-observable antiphase coherences and eliminates them through composite pulse decoupling. We demonstrate the method by isolating signals of an unlabeled post-translational modification tethered to an isotopically enriched protein.

Kenneth A. Marincin et al.

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2021-24', Anonymous Referee #1, 07 Mar 2021
    • AC1: 'Reply on RC1', Kenneth Marincin, 09 Mar 2021
  • RC2: 'Comment on mr-2021-24', Alex Breeze, 10 Mar 2021
    • AC2: 'Reply on RC2', Kenneth Marincin, 22 Mar 2021
      • RC3: 'Reply on AC2', Alex Breeze, 29 Mar 2021

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2021-24', Anonymous Referee #1, 07 Mar 2021
    • AC1: 'Reply on RC1', Kenneth Marincin, 09 Mar 2021
  • RC2: 'Comment on mr-2021-24', Alex Breeze, 10 Mar 2021
    • AC2: 'Reply on RC2', Kenneth Marincin, 22 Mar 2021
      • RC3: 'Reply on AC2', Alex Breeze, 29 Mar 2021

Kenneth A. Marincin et al.

Kenneth A. Marincin et al.

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Short summary
Isotope filters are pivotal for studying interactions between labelled and unlabeled moieties by NMR. We present an isotope filter method to complement existing filters and attenuate undesired signals that escaped them. This advance will readily facilitate molecular studies of binding events or post-translational modifications by NMR.