Preprints
https://doi.org/10.5194/mr-2021-38
https://doi.org/10.5194/mr-2021-38

  08 Apr 2021

08 Apr 2021

Review status: this preprint is currently under review for the journal MR.

A novel multinuclear solid state NMR approach for the characterization of kidney stones

César Leroy1,5, Laure Bonhomme-Coury1, Christel Gervais1, Frederik Tielens1,8, Florence Babonneau1, Michel Daudon2, Dominique Bazin3,4, Emmanuel Letavernier2, Danielle Laurencin5, Dinu Iuga6, John Vincent Hanna6, Mark Edmund Smith7, and Christian Bonhomme1 César Leroy et al.
  • 1Sorbonne Université, CNRS, Laboratoire Chimie de la Matière Condensée de Paris, LCMCP, F-75005 Paris, France
  • 2AP-HP, Hôpital Tenon, Explorations Fonctionnelles Multidisciplinaires et INSERM UMRS 1155, Sorbonne Université, Hôpital Tenon, Paris, France
  • 3Institut de Chimie Physique, UMR CNRS 8000, Bâtiment 350, Université Paris Saclay, 91405 Orsay cedex, France
  • 4Laboratoire de Physique des Solides, UMR CNRS 8502, Bâtiment 510, Université Paris-Sud, 91405 Orsay cedex, France
  • 5ICGM, Univ Montpellier, CNRS, ENSCM, Montpellier, France
  • 6Department of Physics, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, United Kingdom
  • 7Department of Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom
  • 8General Chemistry (ALGC) – Materials Modelling Group, Vrije Universiteit Brussel (Free University Brussels – VUB), Pleinlaan 2, 1050 Brussel, Belgium

Abstract. The spectroscopic study of pathological calcifications (including kidney stones) is extremely rich and helps to improve the understanding of the physical and chemical processes associated with their formation. While FTIR imaging and optical/electron microscopies are routine techniques in hospitals, there has been a dearth of solid state NMR studies introduced into this area of medical research, probably due to the scarcity of this analytical technique in hospital facilities. This work introduces effective multinuclear and multi-dimensional solid state NMR methodologies to study the complex chemical and structural properties characterising kidney stone composition. As a basis for comparison three hydrates (n = 1, 2 and 3) of calcium oxalate are examined along with nine representative kidney stones. The multinuclear MAS NMR approach adopted investigates the 1H, 13C, 31P and 43Ca nuclei, with the 1H and 13C MAS NMR data able to be readily deconvoluted into the constituent elements associated with the different oxalates and organics present. For the first time, the full interpretation of highly resolved 1H NMR spectra is presented for the three hydrates, based on structure and local dynamics. The corresponding 31P MAS NMR data indicates the presence of low-level inorganic phosphate species, however the complexity of these data make the precise identification of the phases difficult to assign. This work provides physicians, urologists and nephrologists with additional avenues of spectroscopic investigation to interrogate this complex medical dilemma that requires real multi technique approaches to generate effective outcomes.

César Leroy et al.

Status: open (until 06 May 2021)

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César Leroy et al.

César Leroy et al.

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Short summary
Kidney stones (KS) are a major health problem in industrialized countries. The study of KS is presently at the heart of a concerted multi-disciplinary axis of research involving physicians, physical chemists and spectroscopists. In this contribution, an in-depth structural description of kidney stones is proposed by implementing a combination of multinuclear and multidimensional solid state NMR methodology.