Preprints
https://doi.org/10.5194/mr-2021-33
https://doi.org/10.5194/mr-2021-33

  26 Mar 2021

26 Mar 2021

Review status: this preprint is currently under review for the journal MR.

129Xe Ultrafast Z-spectroscopy enables micromolar detection of biosensors on a 1T benchtop spectrometer

Kévin Chighine, Estelle Léonce, Céline Boutin, Hervé Desvaux, and Patrick Berthault Kévin Chighine et al.
  • Université Paris-Saclay, CNRS, CEA, Nanosciences et Innovation pour les Matériaux, la Biomédecine et l’Energie, 91191, Gif-sur-Yvette, France

Abstract. The availability of a benchtop NMR spectrometer, of low cost and easily transportable, can allow detection of low quantities of biosensors, provided that hyperpolarized species are used. Here we show that the micromolar threshold can easily be reached, by employing laser-polarized xenon and cage-molecules reversibly hosting it. Indirect detection of caged xenon is made via chemical exchange, using ultrafast Z-spectroscopy based on spatio-temporal encoding. On this non-dedicated low-field spectrometer, several ideas are proposed to improve the signal.

Kévin Chighine et al.

Status: open (until 28 Apr 2021)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse

Kévin Chighine et al.

Kévin Chighine et al.

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Short summary
The use of hyperpolarized species - i.e. species whose nuclear polarization is transiently multiplied by several orders of magnitude - can perfectly be combined with the use of benchtop NMR spectrometers. In particular, laser-polarized 129Xe, due to its large chemical shift range and the proximity of its resonance frequency to 13C, can be observed on a non-dedicated low-field spectrometer. Detection of low amounts of molecules can be achieved using NMR techniques based on chemical exchange.