Preprints
https://doi.org/10.5194/mr-2021-57
https://doi.org/10.5194/mr-2021-57

  12 Jul 2021

12 Jul 2021

Review status: this preprint is currently under review for the journal MR.

A cryogen-free, semi-automated apparatus for bullet-dynamic nuclear polarization with improved resolution

Karel Kouřil1, Michel Gramberg1, Michael Jurkutat1, Hana Kouřilová1, and Benno Meier1,2 Karel Kouřil et al.
  • 1Institute for Biological Interfaces 4, Karlsruhe Institute of Technology, Germany
  • 2Institute of Organic Chemistry, Karlsruhe Institute of Technology, Germany

Abstract. In dissolution-dynamic nuclear polarization, a hyperpolarized solid is dissolved with a jet of hot solvent. The solution is then transferred to a secondary magnet, where spectra can be recorded with improved sensitivity. In bullet-dynamic nuclear polarization this order is reversed. Pressurized gas is used to rapidly transfer the hyperpolarized solid to the secondary magnet, and the hyperpolarized solid is dissolved only upon arrival. A potential advantage of this approach is that it may avoid excessive dilution and the associated signal loss, in particular for small sample quantities. Previously, we have shown that liquid-state NMR spectra with polarization levels of up to 30 % may be recorded within less than 1 second after the departure of the hyperpolarized solid from the polarizing magnet. The resolution of the recorded spectra however was limited. The system consumed substantial amounts of liquid helium and substantial manual work was required in between experiments to prepare for the next shot. Here, we present a new bullet-DNP system that addresses these limitations.

Karel Kouřil et al.

Status: final response (author comments only)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2021-57', Anonymous Referee #1, 05 Aug 2021
  • RC2: 'Comment on mr-2021-57', Anonymous Referee #2, 05 Sep 2021

Karel Kouřil et al.

Karel Kouřil et al.

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Short summary
The signals that are attainable in conventional NMR are tiny and consequentially, NMR is often a slow and insensitive. The intensity may be increased by hyperpolarization, provided that the spectral quality is essentially preserved. We have recently reported a potentially powerful hyperpolarization scheme, named bullet-DNP. Here, we report a 10-fold improvement in resolution for that method, which may enable new applications in metabolomics and drug discovery.