Articles | Volume 2, issue 1
https://doi.org/10.5194/mr-2-15-2021
https://doi.org/10.5194/mr-2-15-2021
Research article
 | 
29 Jan 2021
Research article |  | 29 Jan 2021

Towards resolving the complex paramagnetic nuclear magnetic resonance (NMR) spectrum of small laccase: assignments of resonances to residue-specific nuclei

Rubin Dasgupta, Karthick B. S. S. Gupta, Huub J. M. de Groot, and Marcellus Ubbink

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Latest update: 13 Jun 2024
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Short summary
A method is demonstrated that can help in sequence-specific NMR signal assignment to nuclear spins near a strongly paramagnetic metal in an enzyme. A combination of paramagnetically tailored NMR experiments and second-shell mutagenesis was used to attribute previously observed chemical exchange processes in the active site of laccase to specific histidine ligands. The signals of nuclei close to the metal can be used as spies to unravel the role of motions in the catalytic process.