Articles | Volume 3, issue 2
https://doi.org/10.5194/mr-3-183-2022
© Author(s) 2022. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-3-183-2022
© Author(s) 2022. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
29 Sep 2022
Research article |
| 29 Sep 2022
| 29 Sep 2022
Fine optimization of a dissolution dynamic nuclear polarization experimental setting for 13C NMR of metabolic samples
Arnab Dey
Nantes Université, CNRS, CEISAM UMR 6230, 44000 Nantes, France
Benoît Charrier
Nantes Université, CNRS, CEISAM UMR 6230, 44000 Nantes, France
Karine Lemaitre
Nantes Université, CNRS, CEISAM UMR 6230, 44000 Nantes, France
Victor Ribay
Nantes Université, CNRS, CEISAM UMR 6230, 44000 Nantes, France
Dmitry Eshchenko
Bruker Biospin, Industriestrasse 26, 8117 Fällanden, Switzerland
Marc Schnell
Bruker Biospin, Industriestrasse 26, 8117 Fällanden, Switzerland
Roberto Melzi
Bruker Biospin, Viale V. Lancetti 43, 20158 Milan, Italy
Quentin Stern
Université de Lyon, CNRS, Université Claude Bernard Lyon 1,
ENS de Lyon, Centre de RMN à Très Hauts Champs (CRMN), UMR5082,
69100 Villeurbanne, France
Samuel F. Cousin
Aix Marseille Univ., CNRS, ICR, 13397, Marseille, France
James G. Kempf
Bruker Biospin, 15 Fortune Dr., Billerica, MA 01821 USA
Sami Jannin
Université de Lyon, CNRS, Université Claude Bernard Lyon 1,
ENS de Lyon, Centre de RMN à Très Hauts Champs (CRMN), UMR5082,
69100 Villeurbanne, France
Jean-Nicolas Dumez
Nantes Université, CNRS, CEISAM UMR 6230, 44000 Nantes, France
Patrick Giraudeau
CORRESPONDING AUTHOR
Nantes Université, CNRS, CEISAM UMR 6230, 44000 Nantes, France
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Cited
16 citations as recorded by crossref.
- Recent progress in high-field liquid-state overhauser dynamic nuclear polarization L. Yang et al.
- Possible Applications of Dissolution Dynamic Nuclear Polarization in Conjunction with Zero- to Ultralow-Field Nuclear Magnetic Resonance D. Barskiy et al.
- Benchtop NMR signal enhancement of metabolites in urine extract using SABRE S. Fleischer et al.
- Current state of the art of analyte scope in urine metabolome analysis by non-hydrogenative PHIP N. Reimets et al.
- Hyperpolarized 13C NMR Spectroscopy of Urine Samples at Natural Abundance by Quantitative Dissolution Dynamic Nuclear Polarization V. Ribay et al.
- Quantitative NMR spectroscopy of complex mixtures P. Giraudeau
- Hyperpolarised benchtop NMR spectroscopy for analytical applications A. Silva Terra et al.
- Hyperpolarized 13C NMR Spectroscopy of Urine Samples at Natural Abundance by Quantitative Dissolution Dynamic Nuclear Polarization V. Ribay et al.
- Hyperpolarized 13C NMR Metabolomics of Urine Samples at Natural Abundance Applied to Chronic Kidney Disease V. Ribay et al.
- The Dissolution-Dynamic Nuclear Polarization Experiment B. Meier
- Simultaneous Observation of the Anomerization and Reaction Rates of Enzymatic Dehydrogenation of Glucose-6-Phosphate by Dissolution DNP M. Soussi-Therond et al.
- Hyperpolarized NMR metabolomics V. Ribay et al.
- Hyperpolarized 1H and 13C NMR Spectroscopy in a Single Experiment for Metabolomics A. Dey et al.
- Optimization of heteronuclear ultrafast 2D NMR for the study of complex mixtures hyperpolarized by dynamic nuclear polarization C. Praud et al.
- Boosting 1 H and 13 C NMR signals by orders of magnitude on a bench C. Bocquelet et al.
- Influence of DNP Polarizing Agents on Biochemical Processes: TEMPOL in Transient Ischemic Stroke T. Lê et al.
16 citations as recorded by crossref.
- Recent progress in high-field liquid-state overhauser dynamic nuclear polarization L. Yang et al.
- Possible Applications of Dissolution Dynamic Nuclear Polarization in Conjunction with Zero- to Ultralow-Field Nuclear Magnetic Resonance D. Barskiy et al.
- Benchtop NMR signal enhancement of metabolites in urine extract using SABRE S. Fleischer et al.
- Current state of the art of analyte scope in urine metabolome analysis by non-hydrogenative PHIP N. Reimets et al.
- Hyperpolarized 13C NMR Spectroscopy of Urine Samples at Natural Abundance by Quantitative Dissolution Dynamic Nuclear Polarization V. Ribay et al.
- Quantitative NMR spectroscopy of complex mixtures P. Giraudeau
- Hyperpolarised benchtop NMR spectroscopy for analytical applications A. Silva Terra et al.
- Hyperpolarized 13C NMR Spectroscopy of Urine Samples at Natural Abundance by Quantitative Dissolution Dynamic Nuclear Polarization V. Ribay et al.
- Hyperpolarized 13C NMR Metabolomics of Urine Samples at Natural Abundance Applied to Chronic Kidney Disease V. Ribay et al.
- The Dissolution-Dynamic Nuclear Polarization Experiment B. Meier
- Simultaneous Observation of the Anomerization and Reaction Rates of Enzymatic Dehydrogenation of Glucose-6-Phosphate by Dissolution DNP M. Soussi-Therond et al.
- Hyperpolarized NMR metabolomics V. Ribay et al.
- Hyperpolarized 1H and 13C NMR Spectroscopy in a Single Experiment for Metabolomics A. Dey et al.
- Optimization of heteronuclear ultrafast 2D NMR for the study of complex mixtures hyperpolarized by dynamic nuclear polarization C. Praud et al.
- Boosting 1 H and 13 C NMR signals by orders of magnitude on a bench C. Bocquelet et al.
- Influence of DNP Polarizing Agents on Biochemical Processes: TEMPOL in Transient Ischemic Stroke T. Lê et al.
Saved (final revised paper)
Latest update: 06 May 2026
Short summary
Nuclear magnetic resonance (NMR) is a great tool for analytical chemistry, but it is not the most sensitive one. This work aims to develop a sensitive and repeatable workflow for the analysis of complex samples by hyperpolarized 13C NMR. Through systematic and careful optimization of experimental parameters, we were able to achieve unprecedented sensitivity for the analysis of naturally abundant metabolite mixtures while maintaining the precision required for metabolomic studies.
Nuclear magnetic resonance (NMR) is a great tool for analytical chemistry, but it is not the...
