Magnetic Resonance
Magnetic Resonance
MR
Articles | Volume 4, issue 2
Articles | Volume 4, issue 2
https://doi.org/10.5194/mr-4-187-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-4-187-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 4, issue 2
Research article
 | 
19 Jul 2023
Research article |  | 19 Jul 2023

Cell-free synthesis of proteins with selectively 13C-labelled methyl groups from inexpensive precursors

Damian Van Raad, Gottfried Otting, and Thomas Huber

Viewed

Total article views: 2,265 (including HTML, PDF, and XML)
HTML PDF XML Total Supplement BibTeX EndNote
1,502 703 60 2,265 223 59 82
  • HTML: 1,502
  • PDF: 703
  • XML: 60
  • Total: 2,265
  • Supplement: 223
  • BibTeX: 59
  • EndNote: 82
Views and downloads (calculated since 15 Mar 2023)
Cumulative views and downloads (calculated since 15 Mar 2023)

Viewed (geographical distribution)

Total article views: 2,265 (including HTML, PDF, and XML) Thereof 2,153 with geography defined and 112 with unknown origin.
Country # Views %
  • 1
1
 
 
 
 
Latest update: 02 Dec 2025
Download
Short summary
A novel cell-free protein synthesis system called eCells produces amino acids based on specific isotopes using low-cost precursors. The system selectively labels methyl groups, i.e valine and leucine, with high efficiency. eCells achieve high levels of 13C incorporation and deuteration in protein preparations, making them suitable for NMR experiments of large protein complexes. They are easy to prepare, can be scaled up in volume and are a promising tool for protein production and NMR studies.
Read more
Share