3D-printed microcell for protein NMR at high ionic strengths and small sample volumes

Kakeshpour, Tayeb; Gelenter, Martin D.; Ying, Jinfa; Bax, Ad

doi:https://doi.org/10.5194/mr-6-157-2025

Articles | Volume 6, issue 2

https://doi.org/10.5194/mr-6-157-2025

© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.

https://doi.org/10.5194/mr-6-157-2025

© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 6, issue 2

Research article

|

16 Jul 2025

Research article |

| 16 Jul 2025

3D-printed microcell for protein NMR at high ionic strengths and small sample volumes

Tayeb Kakeshpour, Martin D. Gelenter, Jinfa Ying, and Ad Bax

Data sets

3D-printed microcell for protein NMR at high ionic strengths and small sample volumes (Python scripts) T. Kakeshpour et al. https://doi.org/10.5281/zenodo.15858288

Model code and software

3D-printed microcell for protein NMR at high ionic strengths and small sample volumes (STL file) T. Kakeshpour et al. https://doi.org/10.5281/zenodo.15064675

Short summary

3D-printed sample cells inserted into standard 5 mm NMR (nuclear magnetic resonance) tubes are demonstrated to offer improved sensitivity for biomolecular NMR spectroscopy when the quantity of material is limiting. Using an ellipsoidal shape, magnetic field homogeneity inside the microcell to the first order is insensitive to differences in the magnetic susceptibility of the printer resin and solvent. The sample cells are particularly useful at high ionic strength and at ultrahigh magnetic fields.