Magnetic Resonance
Magnetic Resonance
MR
Articles | Volume 6, issue 2
Articles | Volume 6, issue 2
https://doi.org/10.5194/mr-6-183-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-6-183-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 6, issue 2
Research article
 | 
17 Jul 2025
Research article |  | 17 Jul 2025

Determining large hyperfine interactions of a model flavoprotein in the semiquinone state using pulse EPR (electron paramagnetic resonance) techniques

Jesús I. Martínez, Susana Frago, Milagros Medina, and Inés García-Rubio

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Short summary
Flavoproteins are essential biomolecules involved in many vital processes. Understanding their reactivity requires study of their intermediate semiquinone form. Using advanced spectroscopy and isotopic labeling, we measured key interactions within this state, uncovering subtle changes in the distribution of electron density. These findings refine our understanding of flavoprotein function and highlight the value of combining experiments and computations for studying complex biological systems.
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