Preprints
https://doi.org/10.5194/mr-2024-25
https://doi.org/10.5194/mr-2024-25
24 Jan 2025
 | 24 Jan 2025
Status: this preprint is currently under review for the journal MR.

A fast sample shuttle to couple high and low magnetic fields. Applications to high-resolution relaxometry

Jorge A. Villanueva-Garibay, Andreas Tilch, Ana Paula Aguilar Alva, Guillaume Bouvignies, Frank Engelke, Fabien Ferrage, Agnes Glémot, Ulric B. le Paige, Giulia Licciardi, Claudio Luchinat, Giacomo Parigi, Philippe Pelupessy, Enrico Ravera, Alessandro Ruda, Lucas Siemons, Olof Stenström, and Jean-Max Tyburn

Abstract. Combining high-field high-resolution NMR with an evolution of spin systems at low magnetic field offers many opportunities for the investigation of molecular motions, hyperpolarization and the exploration of field-dependent spin dynamics. Fast and reproducible transfer between high and low fields is required to minimize polarization losses due to longitudinal relaxation. Here, we introduce a new design of a sample shuttle that achieves remarkably high speeds, vmax ~ 27 m.s-1. This hybrid pneumatic/mechanical apparatus is compatible with conventional probes at the high-field center. We show applications to water relaxometry in solutions of paramagnetic ions, high-resolution proton relaxometry of a small molecule and sample shuttling of a solution of a 42 kDa protein. Importantly, this fast sample shuttle system is narrow, with a diameter d = 6 mm for the sample shuttle container based on a standard 5 mm outer diameter glass tube, which should allow near access to the sample for magnetic manipulation at low field.

Competing interests: JAVG and AG are employees, FE is a consultant for, JMT is a former employee and AT an employee of a subcontractor of Bruker Biospin.

Publisher's note: Copernicus Publications remains neutral with regard to jurisdictional claims made in the text, published maps, institutional affiliations, or any other geographical representation in this preprint. The responsibility to include appropriate place names lies with the authors.
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Jorge A. Villanueva-Garibay, Andreas Tilch, Ana Paula Aguilar Alva, Guillaume Bouvignies, Frank Engelke, Fabien Ferrage, Agnes Glémot, Ulric B. le Paige, Giulia Licciardi, Claudio Luchinat, Giacomo Parigi, Philippe Pelupessy, Enrico Ravera, Alessandro Ruda, Lucas Siemons, Olof Stenström, and Jean-Max Tyburn

Status: open (until 21 Feb 2025)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2024-25', Anonymous Referee #1, 29 Jan 2025 reply
  • RC2: 'Comment on mr-2024-25', Anonymous Referee #2, 30 Jan 2025 reply
    • CC1: 'Reply on RC2', Jorge A. Villanueva-Garibay, 12 Feb 2025 reply
Jorge A. Villanueva-Garibay, Andreas Tilch, Ana Paula Aguilar Alva, Guillaume Bouvignies, Frank Engelke, Fabien Ferrage, Agnes Glémot, Ulric B. le Paige, Giulia Licciardi, Claudio Luchinat, Giacomo Parigi, Philippe Pelupessy, Enrico Ravera, Alessandro Ruda, Lucas Siemons, Olof Stenström, and Jean-Max Tyburn
Jorge A. Villanueva-Garibay, Andreas Tilch, Ana Paula Aguilar Alva, Guillaume Bouvignies, Frank Engelke, Fabien Ferrage, Agnes Glémot, Ulric B. le Paige, Giulia Licciardi, Claudio Luchinat, Giacomo Parigi, Philippe Pelupessy, Enrico Ravera, Alessandro Ruda, Lucas Siemons, Olof Stenström, and Jean-Max Tyburn

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Short summary
Investigating NMR phenomena at variable magnetic fields is useful and insightful, in particular for hyperpolarization and molecular dynamics. To benefit from high-resolution at high magnetic fields, field-dependent investigations can be performed on a high-field NMR spectrometer, using a sample shuttle apparatus for field cycling. Here we introduce a new design of sample shuttle, which is fast, reliable, and narrow. We show a series of applications to small molecules and a protein in solution.
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