Articles | Volume 1, issue 1
https://doi.org/10.5194/mr-1-75-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-1-75-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
15 May 2020
Research article |
| 15 May 2020
| 15 May 2020
Distance measurement between trityl radicals by pulse dressed electron paramagnetic resonance with phase modulation
Department of Chemistry and Applied Biosciences, Laboratory of Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093 Zurich, Switzerland
Henrik Hintz
Faculty of Chemistry and Center for Molecular Materials (CM), Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld, Germany
Agathe Vanas
Department of Chemistry and Applied Biosciences, Laboratory of Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093 Zurich, Switzerland
Adelheid Godt
Faculty of Chemistry and Center for Molecular Materials (CM), Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld, Germany
Gunnar Jeschke
Department of Chemistry and Applied Biosciences, Laboratory of Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093 Zurich, Switzerland
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Double electron–electron resonance (DEER) provides information on the structure of proteins by attaching two spin labels to the protein at a well-defined location and measuring the distance between them. The sensitivity of the method in terms of the amount of the protein that is needed for the experiment depends strongly on the relaxation properties of the spin label and the composition of the solvent. We show how to set up the experiment for best sensitivity when the solvent is water (H2O).
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Short summary
Measuring distances between unpaired electron spins is an important application of electron paramagnetic resonance. The longest distance that is accessible is limited by the phase memory time of the electron spins. Here we show that strong continuous microwave irradiation can significantly slow down relaxation. Additionally, we introduce a phase-modulation scheme that allows measurement of the distance during the irradiation. Our approach could thus significantly extend the accessible distances.
Measuring distances between unpaired electron spins is an important application of electron...
