Four-dimensional NOE-NOE spectroscopy of SARS-CoV-2 Main Protease to facilitate resonance assignment and structural analysis
Angus J. Robertson,Jinfa Ying,and Ad Bax
Angus J. Robertson
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Jinfa Ying
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Viewed
Total article views: 2,234 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
1,250
925
59
2,234
424
44
52
HTML: 1,250
PDF: 925
XML: 59
Total: 2,234
Supplement: 424
BibTeX: 44
EndNote: 52
Views and downloads (calculated since 22 Feb 2021)
Cumulative views and downloads
(calculated since 22 Feb 2021)
Total article views: 1,435 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
797
591
47
1,435
247
43
50
HTML: 797
PDF: 591
XML: 47
Total: 1,435
Supplement: 247
BibTeX: 43
EndNote: 50
Views and downloads (calculated since 13 Apr 2021)
Cumulative views and downloads
(calculated since 13 Apr 2021)
Total article views: 799 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
453
334
12
799
177
1
2
HTML: 453
PDF: 334
XML: 12
Total: 799
Supplement: 177
BibTeX: 1
EndNote: 2
Views and downloads (calculated since 22 Feb 2021)
Cumulative views and downloads
(calculated since 22 Feb 2021)
Viewed (geographical distribution)
Total article views: 2,234 (including HTML, PDF, and XML)
Thereof 2,092 with geography defined
and 142 with unknown origin.
Total article views: 1,435 (including HTML, PDF, and XML)
Thereof 1,330 with geography defined
and 105 with unknown origin.
Total article views: 799 (including HTML, PDF, and XML)
Thereof 762 with geography defined
and 37 with unknown origin.
NMR study of large proteins such as SARS-CoV-2 Main Protease can be challenging when exchange broadening, multiple stable conformations, and back-exchanging the fully deuterated chain pose problems. We demonstrate that 4D NMR, including an extension of 3D NOE-NOE spectroscopy, provides an effective tool for spectral analysis. In combination with X-ray coordinates, the 4D NMR data are particularly useful for extending and validating assignments and for probing structural features.
NMR study of large proteins such as SARS-CoV-2 Main Protease can be challenging when exchange...