Articles | Volume 2, issue 1
Research article
13 Apr 2021
Research article |  | 13 Apr 2021

Four-dimensional NOE-NOE spectroscopy of SARS-CoV-2 Main Protease to facilitate resonance assignment and structural analysis

Angus J. Robertson, Jinfa Ying, and Ad Bax


Interactive discussion

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2021-19', Anonymous Referee #1, 25 Feb 2021
  • RC2: 'Comment on mr-2021-19', Anonymous Referee #2, 28 Feb 2021
  • RC3: 'Comment on mr-2021-19', Anonymous Referee #3, 28 Feb 2021
  • RC4: 'Comment on mr-2021-19', Anonymous Referee #4, 05 Mar 2021
  • AC1: 'Comment on mr-2021-19', Ad Bax, 26 Mar 2021

Peer review completion

AR: Author's response | RR: Referee report | ED: Editor decision
AR by Ad Bax on behalf of the Authors (26 Mar 2021)  Author's response
ED: Publish as is (02 Apr 2021) by Isabella Felli
AR by Ad Bax on behalf of the Authors (02 Apr 2021)  Author's response    Manuscript
Short summary
NMR study of large proteins such as SARS-CoV-2 Main Protease can be challenging when exchange broadening, multiple stable conformations, and back-exchanging the fully deuterated chain pose problems. We demonstrate that 4D NMR, including an extension of 3D NOE-NOE spectroscopy, provides an effective tool for spectral analysis. In combination with X-ray coordinates, the 4D NMR data are particularly useful for extending and validating assignments and for probing structural features.