Characterization of nucleosome sediments for protein interaction studies by solid-state NMR spectroscopy
Ulric B. le Paige,ShengQi Xiang,Marco M. R. M. Hendrix,Yi Zhang,Gert E. Folkers,Markus Weingarth,Alexandre M. J. J. Bonvin,Tatiana G. Kutateladze,Ilja K. Voets,Marc Baldus,and Hugo van Ingen
Ulric B. Paige
Utrecht NMR Group, Bijvoet Centre for Biomolecular Research,
Utrecht University, 3584 CH, Utrecht, the Netherlands
ShengQi Xiang
Utrecht NMR Group, Bijvoet Centre for Biomolecular Research,
Utrecht University, 3584 CH, Utrecht, the Netherlands
current address: MOE Key Lab for Membrane-less Organelles &
Cellular Dynamics, School of Life Sciences, University of Science and
Technology of China, 96 Jinzhai Road, Hefei, 230026, Anhui, China
Marco M. R. M. Hendrix
Laboratory of Self-Organizing Soft Matter, Department of Chemical
Engineering and Chemistry & Institute for Complex Molecular Systems,
Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, the
Netherlands
Yi Zhang
Department of Pharmacology, University of Colorado School of
Medicine, Aurora, CO 80045, USA
Gert E. Folkers
Utrecht NMR Group, Bijvoet Centre for Biomolecular Research,
Utrecht University, 3584 CH, Utrecht, the Netherlands
Markus Weingarth
Utrecht NMR Group, Bijvoet Centre for Biomolecular Research,
Utrecht University, 3584 CH, Utrecht, the Netherlands
Alexandre M. J. J. Bonvin
Utrecht NMR Group, Bijvoet Centre for Biomolecular Research,
Utrecht University, 3584 CH, Utrecht, the Netherlands
Tatiana G. Kutateladze
Department of Pharmacology, University of Colorado School of
Medicine, Aurora, CO 80045, USA
Ilja K. Voets
Laboratory of Self-Organizing Soft Matter, Department of Chemical
Engineering and Chemistry & Institute for Complex Molecular Systems,
Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, the
Netherlands
Marc Baldus
Utrecht NMR Group, Bijvoet Centre for Biomolecular Research,
Utrecht University, 3584 CH, Utrecht, the Netherlands
NMR studies can be of great help in understanding the molecular mechanisms of nucleosome functions. For solid-state NMR, nucleosomes need to be tightly packed together. We show that centrifugation of nucleosomes results in formation of gels with very high packing ratios yet without pronounced order in the packing and without formation of specific or stable inter-nucleosome contacts. This makes the approach suitable also for the study of proteins that bind weakly to the nucleosome.
NMR studies can be of great help in understanding the molecular mechanisms of nucleosome...
