Articles | Volume 2, issue 1
https://doi.org/10.5194/mr-2-291-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Special issue:
https://doi.org/10.5194/mr-2-291-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Real-time nuclear magnetic resonance spectroscopy in the study of biomolecular kinetics and dynamics
György Pintér
Institute for Organic Chemistry and Chemical Biology, Center for
Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang
Goethe-Universität Frankfurt, Frankfurt 60438, Germany
Katharina F. Hohmann
Institute for Organic Chemistry and Chemical Biology, Center for
Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang
Goethe-Universität Frankfurt, Frankfurt 60438, Germany
J. Tassilo Grün
Institute for Organic Chemistry and Chemical Biology, Center for
Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang
Goethe-Universität Frankfurt, Frankfurt 60438, Germany
Julia Wirmer-Bartoschek
Institute for Organic Chemistry and Chemical Biology, Center for
Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang
Goethe-Universität Frankfurt, Frankfurt 60438, Germany
Clemens Glaubitz
Institute for Biophysical Chemistry, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-Universität Frankfurt, Frankfurt 60438, Germany
Boris Fürtig
Institute for Organic Chemistry and Chemical Biology, Center for
Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang
Goethe-Universität Frankfurt, Frankfurt 60438, Germany
Harald Schwalbe
CORRESPONDING AUTHOR
Institute for Organic Chemistry and Chemical Biology, Center for
Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang
Goethe-Universität Frankfurt, Frankfurt 60438, Germany
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Cited
15 citations as recorded by crossref.
- Molecular recognition and structural plasticity in amyloid–nucleic acid complexes R. Kukreja & M. Latham https://doi.org/10.1016/j.jsb.2025.108233
- Initial Primer Synthesis of a DNA Primase Monitored by Real-Time NMR Spectroscopy P. Wu et al. https://doi.org/10.1021/jacs.3c11836
- Advances in Cellular and Molecular Biology Assays: A Review of Gold Standard Methods K. Kittelson et al. https://doi.org/10.38124/ijisrt/25mar736
- Navigating the landscape of protein folding and proteostasis: from molecular chaperones to therapeutic innovations O. Kuzu et al. https://doi.org/10.1038/s41392-025-02439-w
- Tiny but multi-stable: four distinct conformational states govern the ligand-free state of the preQ1 riboswitch from a thermophilic bacterium S. Rückriegel et al. https://doi.org/10.1093/nar/gkaf560
- The key role of the dynamics and flexibility of proteins in functional mechanisms: How computational methods can contribute to their identification R. Leon Foun Lin et al. https://doi.org/10.1016/j.biochi.2025.10.007
- Modeling difference x-ray scattering observations from an integral membrane protein within a detergent micelle D. Sarabi et al. https://doi.org/10.1063/4.0000157
- Folding dynamics of polymorphic G‐quadruplex structures J. Grün & H. Schwalbe https://doi.org/10.1002/bip.23477
- Contrasting effects of different molecular crowding environments on base‐pair opening/closing dynamics of DNA triplex structures T. Sakamoto et al. https://doi.org/10.1111/febs.70503
- Refolding of the Four Helical Fragments of Barstar on Gold Nanoparticles X. Zhang et al. https://doi.org/10.1002/cmdc.70411
- An overview of structural approaches to study therapeutic RNAs L. Mollica et al. https://doi.org/10.3389/fmolb.2022.1044126
- Nanosecond Hyperquenching for Electron Cryo‐Microscopy Without Air‐Water Interface Artifacts U. Ermel et al. https://doi.org/10.1002/chem.202403878
- Deamidation and isomerization to elusive isoaspartate in a computationally designed miniprotein K. Blejec & C. Smith https://doi.org/10.1002/pro.70508
- Integrative Real-Time NMR and X-ray Crystallography Reveal Prostaglandin D2 Metabolism S. Ohta et al. https://doi.org/10.1021/acs.analchem.5c03604
- Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes P. Kumar et al. https://doi.org/10.1016/j.biotechadv.2023.108219
15 citations as recorded by crossref.
- Molecular recognition and structural plasticity in amyloid–nucleic acid complexes R. Kukreja & M. Latham https://doi.org/10.1016/j.jsb.2025.108233
- Initial Primer Synthesis of a DNA Primase Monitored by Real-Time NMR Spectroscopy P. Wu et al. https://doi.org/10.1021/jacs.3c11836
- Advances in Cellular and Molecular Biology Assays: A Review of Gold Standard Methods K. Kittelson et al. https://doi.org/10.38124/ijisrt/25mar736
- Navigating the landscape of protein folding and proteostasis: from molecular chaperones to therapeutic innovations O. Kuzu et al. https://doi.org/10.1038/s41392-025-02439-w
- Tiny but multi-stable: four distinct conformational states govern the ligand-free state of the preQ1 riboswitch from a thermophilic bacterium S. Rückriegel et al. https://doi.org/10.1093/nar/gkaf560
- The key role of the dynamics and flexibility of proteins in functional mechanisms: How computational methods can contribute to their identification R. Leon Foun Lin et al. https://doi.org/10.1016/j.biochi.2025.10.007
- Modeling difference x-ray scattering observations from an integral membrane protein within a detergent micelle D. Sarabi et al. https://doi.org/10.1063/4.0000157
- Folding dynamics of polymorphic G‐quadruplex structures J. Grün & H. Schwalbe https://doi.org/10.1002/bip.23477
- Contrasting effects of different molecular crowding environments on base‐pair opening/closing dynamics of DNA triplex structures T. Sakamoto et al. https://doi.org/10.1111/febs.70503
- Refolding of the Four Helical Fragments of Barstar on Gold Nanoparticles X. Zhang et al. https://doi.org/10.1002/cmdc.70411
- An overview of structural approaches to study therapeutic RNAs L. Mollica et al. https://doi.org/10.3389/fmolb.2022.1044126
- Nanosecond Hyperquenching for Electron Cryo‐Microscopy Without Air‐Water Interface Artifacts U. Ermel et al. https://doi.org/10.1002/chem.202403878
- Deamidation and isomerization to elusive isoaspartate in a computationally designed miniprotein K. Blejec & C. Smith https://doi.org/10.1002/pro.70508
- Integrative Real-Time NMR and X-ray Crystallography Reveal Prostaglandin D2 Metabolism S. Ohta et al. https://doi.org/10.1021/acs.analchem.5c03604
- Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes P. Kumar et al. https://doi.org/10.1016/j.biotechadv.2023.108219
Saved (final revised paper)
Latest update: 08 Aug 2026
Short summary
The folding, refolding and misfolding of biomacromolecules including proteins, DNA and RNA is an important area of biophysical research to understand functional and disease states of a cell. NMR spectroscopy provides detailed insight, with both high time and atomic resolution. These experiments put stringent requirements on signal-to-noise for often irreversible folding reactions. The review describes methodological approaches and highlights key applications.
The folding, refolding and misfolding of biomacromolecules including proteins, DNA and RNA is an...
Special issue