Articles | Volume 2, issue 1
https://doi.org/10.5194/mr-2-387-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Special issue:
https://doi.org/10.5194/mr-2-387-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
04 Jun 2021
Research article |
| 04 Jun 2021
| 04 Jun 2021
A novel sample handling system for dissolution dynamic nuclear polarization experiments
Thomas Kress
Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road,
Cambridge CB2 1EW, UK
Kateryna Che
Faculty of Chemistry, Institute of Biological
Chemistry, University of Vienna, Währinger Str. 38, Vienna, Austria
Ludovica M. Epasto
Faculty of Chemistry, Institute of Biological
Chemistry, University of Vienna, Währinger Str. 38, Vienna, Austria
Fanny Kozak
Faculty of Chemistry, Institute of Biological
Chemistry, University of Vienna, Währinger Str. 38, Vienna, Austria
Mattia Negroni
Faculty of Chemistry, Institute of Biological
Chemistry, University of Vienna, Währinger Str. 38, Vienna, Austria
Gregory L. Olsen
Faculty of Chemistry, Institute of Biological
Chemistry, University of Vienna, Währinger Str. 38, Vienna, Austria
Albina Selimovic
Faculty of Chemistry, Institute of Biological
Chemistry, University of Vienna, Währinger Str. 38, Vienna, Austria
Dennis Kurzbach
CORRESPONDING AUTHOR
Faculty of Chemistry, Institute of Biological
Chemistry, University of Vienna, Währinger Str. 38, Vienna, Austria
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Cited
13 citations as recorded by crossref.
- Short-lived calcium carbonate precursors observed in situ via Bullet-dynamic nuclear polarization E. Turhan et al. 10.1038/s42004-024-01300-5
- Real-Time Cell Analysis Using Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy at Single and Bulk Cellular Levels S. Pawar et al. 10.1080/10408347.2025.2572754
- Fine optimization of a dissolution dynamic nuclear polarization experimental setting for 13C NMR of metabolic samples A. Dey et al. 10.5194/mr-3-183-2022
- Toward protein NMR at physiological concentrations by hyperpolarized water—Finding and mapping uncharted conformational spaces L. Epasto et al. 10.1126/sciadv.abq5179
- Inversion of Hyperpolarized 13C NMR Signals through Cross-Correlated Cross-Relaxation in Dissolution DNP Experiments M. Negroni et al. 10.1021/acs.jpcb.2c03375
- Nuclear Overhauser spectroscopy in hyperpolarized water – chemical vs. magnetic exchange L. Epasto et al. 10.1039/D2CC03735A
- Unified understanding of the breakdown of thermal mixing dynamic nuclear polarization: The role of temperature and radical concentration L. Epasto et al. 10.1016/j.jmr.2024.107670
- Frémy’s Salt as a Low-Persistence Hyperpolarization Agent: Efficient Dynamic Nuclear Polarization Plus Rapid Radical Scavenging M. Negroni et al. 10.1021/jacs.2c07960
- Introduction to “Geoffrey Bodenhausen Festschrift” D. Abergel & F. Ferrage 10.5194/mr-4-111-2023
- Hyperpolarized NMR Combined with Quantum Mechanical Simulations Reveal Atomistic Structures of Calcium Phosphate Prenucleation Clusters C. Pötzl et al. 10.1021/acs.analchem.5c02945
- Hyperpolarized water as universal sensitivity booster in biomolecular NMR C. Hilty et al. 10.1038/s41596-022-00693-8
- Biphasic NMR of Hyperpolarized Suspensions─Real-Time Monitoring of Solute-to-Solid Conversion to Watch Materials Grow E. Turhan et al. 10.1021/acs.jpcc.3c04198
- A cryogen-free, semi-automated apparatus for bullet-dynamic nuclear polarization with improved resolution K. Kouřil et al. 10.5194/mr-2-815-2021
13 citations as recorded by crossref.
- Short-lived calcium carbonate precursors observed in situ via Bullet-dynamic nuclear polarization E. Turhan et al. 10.1038/s42004-024-01300-5
- Real-Time Cell Analysis Using Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy at Single and Bulk Cellular Levels S. Pawar et al. 10.1080/10408347.2025.2572754
- Fine optimization of a dissolution dynamic nuclear polarization experimental setting for 13C NMR of metabolic samples A. Dey et al. 10.5194/mr-3-183-2022
- Toward protein NMR at physiological concentrations by hyperpolarized water—Finding and mapping uncharted conformational spaces L. Epasto et al. 10.1126/sciadv.abq5179
- Inversion of Hyperpolarized 13C NMR Signals through Cross-Correlated Cross-Relaxation in Dissolution DNP Experiments M. Negroni et al. 10.1021/acs.jpcb.2c03375
- Nuclear Overhauser spectroscopy in hyperpolarized water – chemical vs. magnetic exchange L. Epasto et al. 10.1039/D2CC03735A
- Unified understanding of the breakdown of thermal mixing dynamic nuclear polarization: The role of temperature and radical concentration L. Epasto et al. 10.1016/j.jmr.2024.107670
- Frémy’s Salt as a Low-Persistence Hyperpolarization Agent: Efficient Dynamic Nuclear Polarization Plus Rapid Radical Scavenging M. Negroni et al. 10.1021/jacs.2c07960
- Introduction to “Geoffrey Bodenhausen Festschrift” D. Abergel & F. Ferrage 10.5194/mr-4-111-2023
- Hyperpolarized NMR Combined with Quantum Mechanical Simulations Reveal Atomistic Structures of Calcium Phosphate Prenucleation Clusters C. Pötzl et al. 10.1021/acs.analchem.5c02945
- Hyperpolarized water as universal sensitivity booster in biomolecular NMR C. Hilty et al. 10.1038/s41596-022-00693-8
- Biphasic NMR of Hyperpolarized Suspensions─Real-Time Monitoring of Solute-to-Solid Conversion to Watch Materials Grow E. Turhan et al. 10.1021/acs.jpcc.3c04198
- A cryogen-free, semi-automated apparatus for bullet-dynamic nuclear polarization with improved resolution K. Kouřil et al. 10.5194/mr-2-815-2021
Latest update: 05 Nov 2025
Short summary
We present a system for facilitated sample vitrification, melting, and transfer in dissolution dynamic nuclear polarization experiments. The system enables insertion of a sample through an airlock in combination with a dedicated dissolution system that is inserted through the same airlock shortly before the melting event. The cryostat can thus be operated continuously at low temperatures, and the melting process is rapid as no pressurization steps of the cryostat are necessary for dissolution.
We present a system for facilitated sample vitrification, melting, and transfer in dissolution...
Special issue