Articles | Volume 2, issue 1
© Author(s) 2021. This work is distributed underthe Creative Commons Attribution 4.0 License.
Exclusively heteronuclear NMR experiments for the investigation of intrinsically disordered proteins: focusing on proline residues
- Final revised paper (published on 01 Jul 2021)
- Supplement to the final revised paper
- Preprint (discussion started on 12 Apr 2021)
Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor |
: Report abuse
- RC1: 'Comment on mr-2021-37', Anonymous Referee #1, 26 Apr 2021
- RC2: 'Comment on mr-2021-37', Anonymous Referee #2, 30 Apr 2021
- AC1: 'Comment on mr-2021-37', Isabella Felli, 19 May 2021
Peer review completion
AR: Author's response | RR: Referee report | ED: Editor decision
AR by Isabella Felli on behalf of the Authors (20 May 2021)  Author's response Author's tracked changes Manuscript
ED: Publish subject to corrections (02 Jun 2021) by Fabien Ferrage
AR by Isabella Felli on behalf of the Authors (11 Jun 2021)  Author's response Manuscript
This is an interesting manuscript showing application of Proline selective NMR experiments in intrinsicaly disordered proteins. The topic is important since prolines are abundant is this kind of proteins. To achieve the goal the direct 13C detection is used, where the lower sensitivity is compensated by lack of solvent exchange, better dispersion, and direct access to proline residues. The experiments are convincingly demonstrated. The pulse-sequences are described carefully, thus it should allow for repetition of reported results. Authors provide several examples of successful applications and consider their utility.
The article is well and clearly written, and is is certainly interesting for Journal readership. Therefore, I am recommending the paper for publication after a minor revision, see below for details:
The informative value of the article certainly could be improved after adding of a few sentences regarding band-selective homodecoupling intead of IPAP and possible 15N detection.