Articles | Volume 2, issue 1
https://doi.org/10.5194/mr-2-511-2021
https://doi.org/10.5194/mr-2-511-2021
Research article
 | 
01 Jul 2021
Research article |  | 01 Jul 2021

Exclusively heteronuclear NMR experiments for the investigation of intrinsically disordered proteins: focusing on proline residues

Isabella C. Felli, Wolfgang Bermel, and Roberta Pierattelli

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Cited articles

Ahuja, P., Cantrelle, F. X., Huvent, I., Hanoulle, X., Lopez, J., Smet, C., Wieruszeski, J. M., Landrieu, I., and Lippens, G.: Proline conformation in a functional Tau fragment, J. Mol. Biol., 428, 79–91, https://doi.org/10.1016/j.jmb.2015.11.023, 2016. 
Alik, A., Bouguechtouli, C., Julien, M., Bermel, W., Ghouil, R., Zinn-Justin, S., and Theillet, F. X.: Sensitivity-Enhanced 13C-NMR spectroscopy for monitoring multisite phosphorylation at physiological temperature and pH, Angew. Chem. Int. Edit., 59, 10411–10415, https://doi.org/10.1002/anie.202002288, 2020. 
Bermel, W., Bertini, I., Felli, I. C., Kümmerle, R., and Pierattelli, R.: Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins, J. Magn. Reson., 178, 56–64, https://doi.org/10.1016/j.jmr.2005.08.011, 2006a. 
Bermel, W., Bertini, I., Felli, I. C., Lee, Y.-M., Luchinat, C., and Pierattelli, R.: Protonless NMR experiments for sequence-specific assignment of backbone nuclei in unfolded proteins, J. Am. Chem. Soc., 128, 3918–3919, https://doi.org/10.1021/ja0582206, 2006b. 
Bermel, W., Bertini, I., Csizmok, V., Felli, I. C., Pierattelli, R., and Tompa, P.: H-start for exclusively heteronuclear NMR spectroscopy: The case of intrinsically disordered proteins, J. Magn. Reson., 198, 275–281, https://doi.org/10.1016/j.jmr.2009.02.012, 2009. 
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Short summary
NMR represents a key spectroscopic technique for studying intrinsically disordered proteins (IDPs) that lack a stable three-dimensional structure. We present a set of NMR experiments tailored for proline residues, which are highly abundant in IDPs. The novel experiments have very interesting properties for the investigation of IDPs of increasing complexity.