Articles | Volume 2, issue 2
Research article
14 Sep 2021
Research article |  | 14 Sep 2021

Rapid assessment of Watson–Crick to Hoogsteen exchange in unlabeled DNA duplexes using high-power SELOPE imino 1H CEST

Bei Liu, Atul Rangadurai, Honglue Shi, and Hashim M. Al-Hashimi


Interactive discussion

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2021-58', Anonymous Referee #1, 02 Aug 2021
    • AC4: 'Reply to RC1', Bei Liu, 19 Aug 2021
  • RC2: 'Comment on mr-2021-58', Anonymous Referee #2, 05 Aug 2021
    • AC5: 'reply to RC2', Bei Liu, 19 Aug 2021

Peer review completion

AR: Author's response | RR: Referee report | ED: Editor decision
AR by Bei Liu on behalf of the Authors (20 Aug 2021)  Author's response    Author's tracked changes    Manuscript
ED: Publish as is (05 Sep 2021) by Fabien Ferrage
Short summary
There is growing interest in mapping exchange dynamics between Watson–Crick and Hoogsteen conformations across different DNA contexts. However, current methods are ill-suited for measurements at a large scale because they require isotopically enriched samples. We report that Hoogsteen dynamics can be measured on unlabeled samples using 1H CEST experiments, which have higher throughput and lower cost relative to conventional methods and also provide new insights into Hoogsteen dynamics.