Magnetic Resonance
Magnetic Resonance
MR
Articles | Volume 3, issue 1
Articles | Volume 3, issue 1
https://doi.org/10.5194/mr-3-65-2022
© Author(s) 2022. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-3-65-2022
© Author(s) 2022. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 3, issue 1
Research article
 | 
09 May 2022
Research article |  | 09 May 2022

Localising nuclear spins by pseudocontact shifts from a single tagging site

Henry W. Orton, Elwy H. Abdelkader, Lydia Topping, Stephen J. Butler, and Gottfried Otting

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Latest update: 13 Dec 2024
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Short summary
Installing a tag containing a paramagnetic metal ion on a protein can lead to large changes (pseudocontact shifts) in the resonances observed in NMR spectra. These are easily measured and contain valuable long-range structural information. The present work shows that a single tagging site furnished with different tags can be sufficient to localise atoms in proteins with high accuracy. In fact, this strategy works almost as well as the same number of tags distributed over multiple tagging sites.
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