ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Elwy H. Abdelkader
ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Lydia Topping
Department of Chemistry, Loughborough University, Epinal Way, Loughborough, LE11 3TU, United Kingdom
Stephen J. Butler
Department of Chemistry, Loughborough University, Epinal Way, Loughborough, LE11 3TU, United Kingdom
ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Viewed
Total article views: 2,848 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
2,056
713
79
2,848
376
163
138
HTML: 2,056
PDF: 713
XML: 79
Total: 2,848
Supplement: 376
BibTeX: 163
EndNote: 138
Views and downloads (calculated since 23 Feb 2022)
Cumulative views and downloads
(calculated since 23 Feb 2022)
Total article views: 2,073 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
1,573
444
56
2,073
184
149
122
HTML: 1,573
PDF: 444
XML: 56
Total: 2,073
Supplement: 184
BibTeX: 149
EndNote: 122
Views and downloads (calculated since 09 May 2022)
Cumulative views and downloads
(calculated since 09 May 2022)
Total article views: 775 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
483
269
23
775
192
14
16
HTML: 483
PDF: 269
XML: 23
Total: 775
Supplement: 192
BibTeX: 14
EndNote: 16
Views and downloads (calculated since 23 Feb 2022)
Cumulative views and downloads
(calculated since 23 Feb 2022)
Viewed (geographical distribution)
Total article views: 2,848 (including HTML, PDF, and XML)
Thereof 2,644 with geography defined
and 204 with unknown origin.
Total article views: 2,073 (including HTML, PDF, and XML)
Thereof 1,903 with geography defined
and 170 with unknown origin.
Total article views: 775 (including HTML, PDF, and XML)
Thereof 741 with geography defined
and 34 with unknown origin.
Installing a tag containing a paramagnetic metal ion on a protein can lead to large changes (pseudocontact shifts) in the resonances observed in NMR spectra. These are easily measured and contain valuable long-range structural information. The present work shows that a single tagging site furnished with different tags can be sufficient to localise atoms in proteins with high accuracy. In fact, this strategy works almost as well as the same number of tags distributed over multiple tagging sites.
Installing a tag containing a paramagnetic metal ion on a protein can lead to large changes...
