ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Elwy H. Abdelkader
ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Lydia Topping
Department of Chemistry, Loughborough University, Epinal Way, Loughborough, LE11 3TU, United Kingdom
Stephen J. Butler
Department of Chemistry, Loughborough University, Epinal Way, Loughborough, LE11 3TU, United Kingdom
ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Viewed
Total article views: 2,449 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
1,736
647
66
2,449
320
116
102
HTML: 1,736
PDF: 647
XML: 66
Total: 2,449
Supplement: 320
BibTeX: 116
EndNote: 102
Views and downloads (calculated since 23 Feb 2022)
Cumulative views and downloads
(calculated since 23 Feb 2022)
Total article views: 1,729 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
1,295
391
43
1,729
164
102
86
HTML: 1,295
PDF: 391
XML: 43
Total: 1,729
Supplement: 164
BibTeX: 102
EndNote: 86
Views and downloads (calculated since 09 May 2022)
Cumulative views and downloads
(calculated since 09 May 2022)
Total article views: 720 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
441
256
23
720
156
14
16
HTML: 441
PDF: 256
XML: 23
Total: 720
Supplement: 156
BibTeX: 14
EndNote: 16
Views and downloads (calculated since 23 Feb 2022)
Cumulative views and downloads
(calculated since 23 Feb 2022)
Viewed (geographical distribution)
Total article views: 2,449 (including HTML, PDF, and XML)
Thereof 2,252 with geography defined
and 197 with unknown origin.
Total article views: 1,729 (including HTML, PDF, and XML)
Thereof 1,570 with geography defined
and 159 with unknown origin.
Total article views: 720 (including HTML, PDF, and XML)
Thereof 682 with geography defined
and 38 with unknown origin.
Installing a tag containing a paramagnetic metal ion on a protein can lead to large changes (pseudocontact shifts) in the resonances observed in NMR spectra. These are easily measured and contain valuable long-range structural information. The present work shows that a single tagging site furnished with different tags can be sufficient to localise atoms in proteins with high accuracy. In fact, this strategy works almost as well as the same number of tags distributed over multiple tagging sites.
Installing a tag containing a paramagnetic metal ion on a protein can lead to large changes...
