Articles | Volume 3, issue 1
https://doi.org/10.5194/mr-3-65-2022
https://doi.org/10.5194/mr-3-65-2022
Research article
 | 
09 May 2022
Research article |  | 09 May 2022

Localising nuclear spins by pseudocontact shifts from a single tagging site

Henry W. Orton, Elwy H. Abdelkader, Lydia Topping, Stephen J. Butler, and Gottfried Otting

Viewed

Total article views: 1,758 (including HTML, PDF, and XML)
HTML PDF XML Total Supplement BibTeX EndNote
1,290 412 56 1,758 187 43 41
  • HTML: 1,290
  • PDF: 412
  • XML: 56
  • Total: 1,758
  • Supplement: 187
  • BibTeX: 43
  • EndNote: 41
Views and downloads (calculated since 23 Feb 2022)
Cumulative views and downloads (calculated since 23 Feb 2022)

Viewed (geographical distribution)

Total article views: 1,758 (including HTML, PDF, and XML) Thereof 1,576 with geography defined and 182 with unknown origin.
Country # Views %
  • 1
1
 
 
 
 

Cited

Latest update: 20 Nov 2024
Download
Short summary
Installing a tag containing a paramagnetic metal ion on a protein can lead to large changes (pseudocontact shifts) in the resonances observed in NMR spectra. These are easily measured and contain valuable long-range structural information. The present work shows that a single tagging site furnished with different tags can be sufficient to localise atoms in proteins with high accuracy. In fact, this strategy works almost as well as the same number of tags distributed over multiple tagging sites.