Articles | Volume 3, issue 1
https://doi.org/10.5194/mr-3-65-2022
https://doi.org/10.5194/mr-3-65-2022
Research article
 | 
09 May 2022
Research article |  | 09 May 2022

Localising nuclear spins by pseudocontact shifts from a single tagging site

Henry W. Orton, Elwy H. Abdelkader, Lydia Topping, Stephen J. Butler, and Gottfried Otting

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Cited articles

Abdelkader, E. H.: 15N-HSQC spectra of tagged Ubiquitin S57C, Zenodo [data set], https://doi.org/10.5281/zenodo.6004596, 2022. a
Banci, L., Bertini, I., Bren, K. L., Cremonini, M. A., Gray, H. B., Luchinat, C., and Turano, P.: The use of pseudocontact shifts to refine solution structures of paramagnetic metalloproteins: Met80Ala cyano-cytochrome c as an example, J. Biol. Inorg. Chem., 1, 117–126, https://doi.org/10.1007/s007750050030, 1996. a
Bertini, I., Janik, M. B. L., Lee, Y. M., Luchinat, C., and Rosato, A.: Magnetic susceptibility tensor anisotropies for a lanthanide ion series in a fixed protein matrix, J. Am. Chem. Soc., 123, 4181–4188, https://doi.org/10.1021/ja0028626, 2001. a
Brewer, K. D., Bacaj, T., Cavalli, A., Camilloni, C., Swarbrick, J. D., Liu, J., Zhou, A., Zhou, P., Barlow, N., Xu, J., Seven, A. B., Prinslow, E. A., Voleti, R., Häussinger, D., Bonvin, A. M. J. J., Tomchick, D. R., Vendruscolo, M., Graham, B., Südhof, T. C., and Rizo, J.: Dynamic binding mode of a synaptotagmin-1-SNARE complex in solution, Nat. Struct. Mol. Biol., 22, 555–564, https://doi.org/10.1038/nsmb.3035, 2015. a
Chen, W.-N., Nitsche, C., Pilla, K. B., Graham, B., Huber, T., Klein, C. D., and Otting, G.: Sensitive NMR approach for determining the binding mode of tightly binding ligand molecules to protein targets, J. Am. Chem. Soc., 138, 4539–4546, https://doi.org/10.1021/jacs.6b00416, 2016. a, b
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Short summary
Installing a tag containing a paramagnetic metal ion on a protein can lead to large changes (pseudocontact shifts) in the resonances observed in NMR spectra. These are easily measured and contain valuable long-range structural information. The present work shows that a single tagging site furnished with different tags can be sufficient to localise atoms in proteins with high accuracy. In fact, this strategy works almost as well as the same number of tags distributed over multiple tagging sites.