Articles | Volume 5, issue 2
https://doi.org/10.5194/mr-5-95-2024
© Author(s) 2024. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-5-95-2024
© Author(s) 2024. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
NMR side-chain assignments of the Crimean–Congo hemorrhagic fever virus glycoprotein n cytosolic domain
Louis Brigandat
Molecular Microbiology and Structural Biochemistry (MMSB), UMR5086 CNRS, University of Lyon, 7, passage du Vercors, 69367 Lyon, CEDEX 07, France
Maëlys Laux
Molecular Microbiology and Structural Biochemistry (MMSB), UMR5086 CNRS, University of Lyon, 7, passage du Vercors, 69367 Lyon, CEDEX 07, France
Caroline Marteau
Molecular Microbiology and Structural Biochemistry (MMSB), UMR5086 CNRS, University of Lyon, 7, passage du Vercors, 69367 Lyon, CEDEX 07, France
Laura Cole
Molecular Microbiology and Structural Biochemistry (MMSB), UMR5086 CNRS, University of Lyon, 7, passage du Vercors, 69367 Lyon, CEDEX 07, France
Anja Böckmann
Molecular Microbiology and Structural Biochemistry (MMSB), UMR5086 CNRS, University of Lyon, 7, passage du Vercors, 69367 Lyon, CEDEX 07, France
Lauriane Lecoq
Molecular Microbiology and Structural Biochemistry (MMSB), UMR5086 CNRS, University of Lyon, 7, passage du Vercors, 69367 Lyon, CEDEX 07, France
Marie-Laure Fogeron
Molecular Microbiology and Structural Biochemistry (MMSB), UMR5086 CNRS, University of Lyon, 7, passage du Vercors, 69367 Lyon, CEDEX 07, France
Molecular Microbiology and Structural Biochemistry (MMSB), UMR5086 CNRS, University of Lyon, 7, passage du Vercors, 69367 Lyon, CEDEX 07, France
Related authors
No articles found.
Václav Římal, Morgane Callon, Alexander A. Malär, Riccardo Cadalbert, Anahit Torosyan, Thomas Wiegand, Matthias Ernst, Anja Böckmann, and Beat H. Meier
Magn. Reson., 3, 15–26, https://doi.org/10.5194/mr-3-15-2022, https://doi.org/10.5194/mr-3-15-2022, 2022
Short summary
Short summary
Through the advent of fast magic-angle spinning and high magnetic fields, the spectral resolution of solid-state NMR spectra has recently been greatly improved. To take full advantage of this gain, the magnetic field must be stable over the experiment time of hours or even days. We thus monitor the field by simultaneous acquisition of a frequency reference (SAFR) and use this information to correct multidimensional spectra improving resolution and availability of productive magnet time.
Related subject area
Field: Liquid-state NMR | Topic: Applications – biological macromolecules
Facilitating the structural characterisation of non-canonical amino acids in biomolecular NMR
Site-selective generation of lanthanoid binding sites on proteins using 4-fluoro-2,6-dicyanopyridine
Imatinib disassembles the regulatory core of Abelson kinase by binding to its ATP site and not by binding to its myristoyl pocket
Localising nuclear spins by pseudocontact shifts from a single tagging site
Localising individual atoms of tryptophan side chains in the metallo-β-lactamase IMP-1 by pseudocontact shifts from paramagnetic lanthanoid tags at multiple sites
Fluorine NMR study of proline-rich sequences using fluoroprolines
Analysis of conformational exchange processes using methyl-TROSY-based Hahn echo measurements of quadruple-quantum relaxation
Anomalous amide proton chemical shifts as signatures of hydrogen bonding to aromatic sidechains
Rapid assessment of Watson–Crick to Hoogsteen exchange in unlabeled DNA duplexes using high-power SELOPE imino 1H CEST
High-affinity tamoxifen analogues retain extensive positional disorder when bound to calmodulin
Structural polymorphism and substrate promiscuity of a ribosome-associated molecular chaperone
Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling
Real-time nuclear magnetic resonance spectroscopy in the study of biomolecular kinetics and dynamics
The long-standing relationship between paramagnetic NMR and iron–sulfur proteins: the mitoNEET example. An old method for new stories or the other way around?
Conformational features and ionization states of Lys side chains in a protein studied using the stereo-array isotope labeling (SAIL) method
Fragile protein folds: sequence and environmental factors affecting the equilibrium of two interconverting, stably folded protein conformations
Towards resolving the complex paramagnetic nuclear magnetic resonance (NMR) spectrum of small laccase: assignments of resonances to residue-specific nuclei
Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy
Sarah Kuschert, Martin Stroet, Yanni Ka-Yan Chin, Anne Claire Conibear, Xinying Jia, Thomas Lee, Christian Reinhard Otto Bartling, Kristian Strømgaard, Peter Güntert, Karl Johan Rosengren, Alan Edward Mark, and Mehdi Mobli
Magn. Reson., 4, 57–72, https://doi.org/10.5194/mr-4-57-2023, https://doi.org/10.5194/mr-4-57-2023, 2023
Short summary
Short summary
The 20 genetically encoded amino acids provide the basis for most proteins and peptides that make up the machinery of life. This limited repertoire is vastly expanded by the introduction of non-canonical amino acids (ncAAs). Studying the structure of protein-containing ncAAs requires new computational representations that are compatible with existing modelling software. We have developed an online tool for this to aid future structural studies of this class of complex biopolymer.
Sreelakshmi Mekkattu Tharayil, Mithun C. Mahawaththa, Akiva Feintuch, Ansis Maleckis, Sven Ullrich, Richard Morewood, Michael J. Maxwell, Thomas Huber, Christoph Nitsche, Daniella Goldfarb, and Gottfried Otting
Magn. Reson., 3, 169–182, https://doi.org/10.5194/mr-3-169-2022, https://doi.org/10.5194/mr-3-169-2022, 2022
Short summary
Short summary
Having shown that tagging a protein at a single site with different lanthanoid complexes delivers outstanding structural information at a selected site of a protein (such as active sites and ligand binding sites), we now present a simple way by which different lanthanoid complexes can be assembled on a highly solvent-exposed cysteine residue. Furthermore, the chemical assembly is selective for selenocysteine, if a selenocysteine residue can be introduced into the protein of interest.
Stephan Grzesiek, Johannes Paladini, Judith Habazettl, and Rajesh Sonti
Magn. Reson., 3, 91–99, https://doi.org/10.5194/mr-3-91-2022, https://doi.org/10.5194/mr-3-91-2022, 2022
Short summary
Short summary
We show here that binding of the anticancer drug imatinib to the ATP site of Abelson kinase and not binding to its allosteric site coincides with the opening of the kinase regulatory core at nanomolar concentrations. This has implications for the understanding of Abelson’s kinase regulation and activity during medication as well as for the design of new Abelson kinase inhibitors.
Henry W. Orton, Elwy H. Abdelkader, Lydia Topping, Stephen J. Butler, and Gottfried Otting
Magn. Reson., 3, 65–76, https://doi.org/10.5194/mr-3-65-2022, https://doi.org/10.5194/mr-3-65-2022, 2022
Short summary
Short summary
Installing a tag containing a paramagnetic metal ion on a protein can lead to large changes (pseudocontact shifts) in the resonances observed in NMR spectra. These are easily measured and contain valuable long-range structural information. The present work shows that a single tagging site furnished with different tags can be sufficient to localise atoms in proteins with high accuracy. In fact, this strategy works almost as well as the same number of tags distributed over multiple tagging sites.
Henry W. Orton, Iresha D. Herath, Ansis Maleckis, Shereen Jabar, Monika Szabo, Bim Graham, Colum Breen, Lydia Topping, Stephen J. Butler, and Gottfried Otting
Magn. Reson., 3, 1–13, https://doi.org/10.5194/mr-3-1-2022, https://doi.org/10.5194/mr-3-1-2022, 2022
Short summary
Short summary
This paper explores a method for determining the solution structure of a solvent-exposed polypeptide segment (the L3 loop), which is next to the active site of the penicillin-degrading enzyme IMP-1. Tagging three different sites on the protein with paramagnetic metal ions allowed positioning of the L3 loop with atomic resolution. It was found that the method was more robust when omitting data obtained with different metal ions if obtained with the same tag at the same tagging site.
Davy Sinnaeve, Abir Ben Bouzayene, Emile Ottoy, Gert-Jan Hofman, Eva Erdmann, Bruno Linclau, Ilya Kuprov, José C. Martins, Vladimir Torbeev, and Bruno Kieffer
Magn. Reson., 2, 795–813, https://doi.org/10.5194/mr-2-795-2021, https://doi.org/10.5194/mr-2-795-2021, 2021
Short summary
Short summary
Fluorine NMR was used to study the interaction between a proline-rich peptide and a SH3 domain using 4S- and 4R-fluorinated prolines whose potential as NMR probes has not been exploited yet. We present a comprehensive study addressing several aspects to be considered when using these residues as NMR probes, including relaxation and dynamics. We show that their conformational bias may be used to modulate the kinetics of protein binding to proline-rich motifs.
Christopher A. Waudby and John Christodoulou
Magn. Reson., 2, 777–793, https://doi.org/10.5194/mr-2-777-2021, https://doi.org/10.5194/mr-2-777-2021, 2021
Short summary
Short summary
We describe a suite of experiments that exploit field-dependent relaxation measurements of four-spin transitions in methyl groups to characterise chemical exchange processes and which can be used as an alternative or complement to CPMG relaxation dispersion measurements. We show that these four-spin transitions benefit from the methyl TROSY effect and so provide a unique combination of slow intrinsic relaxation and high sensitivity to chemical exchange.
Kumaran Baskaran, Colin W. Wilburn, Jonathan R. Wedell, Leonardus M. I. Koharudin, Eldon L. Ulrich, Adam D. Schuyler, Hamid R. Eghbalnia, Angela M. Gronenborn, and Jeffrey C. Hoch
Magn. Reson., 2, 765–775, https://doi.org/10.5194/mr-2-765-2021, https://doi.org/10.5194/mr-2-765-2021, 2021
Short summary
Short summary
The Biological Magnetic Resonance Data Bank (BMRB) has been used to identify overall trends, for example, the relationship between chemical shift and backbone conformation. The BMRB archive has grown so that statistical outliers are sufficiently numerous to afford insights into unusual or unique structural features in proteins. We analyze amide proton chemical shift outliers to gain insights into the occurrence of hydrogen bonds between an amide NH and the p-pi cloud of aromatic sidechains.
Bei Liu, Atul Rangadurai, Honglue Shi, and Hashim M. Al-Hashimi
Magn. Reson., 2, 715–731, https://doi.org/10.5194/mr-2-715-2021, https://doi.org/10.5194/mr-2-715-2021, 2021
Short summary
Short summary
There is growing interest in mapping exchange dynamics between Watson–Crick and Hoogsteen conformations across different DNA contexts. However, current methods are ill-suited for measurements at a large scale because they require isotopically enriched samples. We report that Hoogsteen dynamics can be measured on unlabeled samples using 1H CEST experiments, which have higher throughput and lower cost relative to conventional methods and also provide new insights into Hoogsteen dynamics.
Lilia Milanesi, Clare R. Trevitt, Brian Whitehead, Andrea M. Hounslow, Salvador Tomas, Laszlo L. P. Hosszu, Christopher A. Hunter, and Jonathan P. Waltho
Magn. Reson., 2, 629–642, https://doi.org/10.5194/mr-2-629-2021, https://doi.org/10.5194/mr-2-629-2021, 2021
Short summary
Short summary
The overall aim of the study is to provide a basis from which to improve the ability of tamoxifen family drugs to reduce the activity of a secondary target protein, calmodulin, during tumour development. The main conclusion is that the binding of a tamoxifen analogue is quite unlike that of other anti-calmodulin compounds in that two drug molecules bring the two domains of calmodulin into close proximity, but they are not fixed in orientation relative to the protein.
Chih-Ting Huang, Yei-Chen Lai, Szu-Yun Chen, Meng-Ru Ho, Yun-Wei Chiang, and Shang-Te Danny Hsu
Magn. Reson., 2, 375–386, https://doi.org/10.5194/mr-2-375-2021, https://doi.org/10.5194/mr-2-375-2021, 2021
Short summary
Short summary
Trigger factor (TF) is a conserved bacterial molecular chaperone that exists in a monomer–dimer equilibrium in solution. It binds to the ribosome as a monomer to facilitate folding of nascent polypeptide chains. We showed that dimeric TF exhibits distinct domain dynamics and conformational polymorphism and that TF contains multiple substrate binding sites that are only accessible in its monomeric form. The equilibrium of TF in different oligomeric states may serve as a regulatory mechanism.
Nestor Kamdem, Yvette Roske, Dmytro Kovalskyy, Maxim O. Platonov, Oleksii Balinskyi, Annika Kreuchwig, Jörn Saupe, Liang Fang, Anne Diehl, Peter Schmieder, Gerd Krause, Jörg Rademann, Udo Heinemann, Walter Birchmeier, and Hartmut Oschkinat
Magn. Reson., 2, 355–374, https://doi.org/10.5194/mr-2-355-2021, https://doi.org/10.5194/mr-2-355-2021, 2021
Short summary
Short summary
The Wnt signalling pathway plays a major role in prevention of cancer, whereby the protein Dishevelled connects from the transmembrane receptor Frizzled to downstream effectors via its PDZ domain. Here, cycles of chemical synthesis and structural biology are applied to develop PDZ ligands that block the Frizzled–Dishevelled interaction using NMR for screening, in ligand development, and for deriving structure–activity relationships. Cellular reporter assays demonstrate their efficacy.
György Pintér, Katharina F. Hohmann, J. Tassilo Grün, Julia Wirmer-Bartoschek, Clemens Glaubitz, Boris Fürtig, and Harald Schwalbe
Magn. Reson., 2, 291–320, https://doi.org/10.5194/mr-2-291-2021, https://doi.org/10.5194/mr-2-291-2021, 2021
Short summary
Short summary
The folding, refolding and misfolding of biomacromolecules including proteins, DNA and RNA is an important area of biophysical research to understand functional and disease states of a cell. NMR spectroscopy provides detailed insight, with both high time and atomic resolution. These experiments put stringent requirements on signal-to-noise for often irreversible folding reactions. The review describes methodological approaches and highlights key applications.
Francesca Camponeschi, Angelo Gallo, Mario Piccioli, and Lucia Banci
Magn. Reson., 2, 203–221, https://doi.org/10.5194/mr-2-203-2021, https://doi.org/10.5194/mr-2-203-2021, 2021
Short summary
Short summary
The iron–sulfur cluster binding properties of human mitoNEET have been investigated by 1D and 2D 1H paramagnetic NMR spectroscopy. The NMR spectra of both oxidized and reduced mitoNEET are significantly different from those reported previously for other [Fe2S2] proteins. Our findings revealed the unique electronic properties of mitoNEET and suggests that the specific electronic structure of the cluster possibly drives the functional properties of different [Fe2S2] proteins.
Mitsuhiro Takeda, Yohei Miyanoiri, Tsutomu Terauchi, and Masatsune Kainosho
Magn. Reson., 2, 223–237, https://doi.org/10.5194/mr-2-223-2021, https://doi.org/10.5194/mr-2-223-2021, 2021
Short summary
Short summary
Although both the hydrophobic aliphatic chain and hydrophilic ζ-amino group of the lysine side chain presumably contribute to the structures and functions of proteins, the dual nature of the lysine residue has not been fully understood yet, due to the lack of appropriate methods to acquire comprehensive information on its long consecutive methylene chain at the atomic scale. We describe herein a novel strategy to address the current situation using nuclear magnetic resonance spectroscopy.
Xingjian Xu, Igor Dikiy, Matthew R. Evans, Leandro P. Marcelino, and Kevin H. Gardner
Magn. Reson., 2, 63–76, https://doi.org/10.5194/mr-2-63-2021, https://doi.org/10.5194/mr-2-63-2021, 2021
Short summary
Short summary
While most proteins adopt one conformation, several interconvert between two or more very different structures. Knowing how sequence changes and small-molecule binding can control this behavior is essential for both understanding biology and inspiring new “molecular switches” which can control cellular pathways. This work contributes by examining these topics in the ARNT protein, showing that features of both the folded and unfolded states contribute to the interconversion process.
Rubin Dasgupta, Karthick B. S. S. Gupta, Huub J. M. de Groot, and Marcellus Ubbink
Magn. Reson., 2, 15–23, https://doi.org/10.5194/mr-2-15-2021, https://doi.org/10.5194/mr-2-15-2021, 2021
Short summary
Short summary
A method is demonstrated that can help in sequence-specific NMR signal assignment to nuclear spins near a strongly paramagnetic metal in an enzyme. A combination of paramagnetically tailored NMR experiments and second-shell mutagenesis was used to attribute previously observed chemical exchange processes in the active site of laccase to specific histidine ligands. The signals of nuclei close to the metal can be used as spies to unravel the role of motions in the catalytic process.
Sreelakshmi Mekkattu Tharayil, Mithun Chamikara Mahawaththa, Choy-Theng Loh, Ibidolapo Adekoya, and Gottfried Otting
Magn. Reson., 2, 1–13, https://doi.org/10.5194/mr-2-1-2021, https://doi.org/10.5194/mr-2-1-2021, 2021
Short summary
Short summary
A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.
Cited articles
Arragain, B., Reguera, J., Desfosses, A., Gutsche, I., Schoehn, G., and Malet, H.: High resolution cryo-EM structure of the helical RNA-bound Hantaan virus nucleocapsid reveals its assembly mechanisms, eLife, 8, e43075, https://doi.org/10.7554/eLife.43075, 2019.
Arragain, B., Effantin, G., Gerlach, P., Reguera, J., Schoehn, G., Cusack, S., and Malet, H.: Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes, Nat. Commun., 11, 3590, https://doi.org/10.1038/s41467-020-17349-4, 2020.
Arragain, B., Durieux Trouilleton, Q., Baudin, F., Provaznik, J., Azevedo, N., Cusack, S., Schoehn, G., and Malet, H.: Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription, Nat. Commun., 13, 902, https://doi.org/10.1038/s41467-022-28428-z, 2022.
Bernard, C., Joly Kukla, C., Rakotoarivony, I., Duhayon, M., Stachurski, F., Huber, K., Giupponi, C., Zortman, I., Holzmuller, P., Pollet, T., Jeanneau, M., Mercey, A., Vachiery, N., Lefrançois, T., Garros, C., Michaud, V., Comtet, L., Despois, L., Pourquier, P., Picard, C., Journeaux, A., Thomas, D., Godard, S., Moissonnier, E., Mely, S., Sega, M., Pannetier, D., Baize, S., and Vial, L.: Detection of Crimean–Congo haemorrhagic fever virus in Hyalomma marginatum ticks, southern France, May 2022 and April 2023, Eurosurveillance, 29, 2400023, https://doi.org/10.2807/1560-7917.ES.2024.29.6.2400023, 2024.
Bignon, E. A., Albornoz, A., Guardado-Calvo, P., Rey, F. A., and Tischler, N. D.: Molecular organization and dynamics of the fusion protein Gc at the hantavirus surface, eLife, 8, e46028, https://doi.org/10.7554/eLife.46028, 2019.
Bonnet, S. I., Vourc'h, G., Raffetin, A., Falchi, A., Figoni, J., Fite, J., Hoch, T., Moutailler, S., and Quillery, E.: The control of Hyalomma ticks, vectors of the Crimean–Congo hemorrhagic fever virus: Where are we now and where are we going?, PLoS Negl. Trop. D, 16, e0010846, https://doi.org/10.1371/journal.pntd.0010846, 2022.
Estrada, D. F. and De Guzman, R. N.: Structural Characterization of the Crimean-Congo Hemorrhagic Fever Virus Gn Tail Provides Insight into Virus Assembly, J. Biol. Chem., 286, 21678–21686, https://doi.org/10.1074/jbc.M110.216515, 2011.
Estrada, D. F., Boudreaux, D. M., Zhong, D., St. Jeor, S. C., and De Guzman, R. N.: The Hantavirus Glycoprotein G1 Tail Contains Dual CCHC-type Classical Zinc Fingers, J. Biol. Chem., 284, 8654–8660, https://doi.org/10.1074/jbc.M808081200, 2009.
Favier, A. and Brutscher, B.: NMRlib: user-friendly pulse sequence tools for Bruker NMR spectrometers, J. Biomol. NMR, 73, 199–211, https://doi.org/10.1007/s10858-019-00249-1, 2019.
Fogeron, M.-L., Badillo, A., Penin, F., and Böckmann, A.: Wheat Germ Cell-Free Overexpression for the Production of Membrane Proteins, in: Membrane Protein Structure and Function Characterization, vol. 1635, edited by: Lacapere, J.-J., Springer New York, New York, NY, 91–108, https://doi.org/10.1007/978-1-4939-7151-0_5, 2017.
Guardado-Calvo, P. and Rey, F. A.: The Envelope Proteins of the Bunyavirales, in: Advances in Virus Research, vol. 98, Elsevier, 83–118, https://doi.org/10.1016/bs.aivir.2017.02.002, 2017.
Halldorsson, S., Li, S., Li, M., Harlos, K., Bowden, T. A., and Huiskonen, J. T.: Shielding and activation of a viral membrane fusion protein, Nat. Commun., 9, 349, https://doi.org/10.1038/s41467-017-02789-2, 2018.
Hastie, K. M., Zandonatti, M. A., Kleinfelter, L. M., Heinrich, M. L., Rowland, M. M., Chandran, K., Branco, L. M., Robinson, J. E., Garry, R. F., and Saphire, E. O.: Structural basis for antibody-mediated neutralization of Lassa virus, Science, 356, 923–928, https://doi.org/10.1126/science.aam7260, 2017.
Hawman, D. W. and Feldmann, H.: Crimean–Congo haemorrhagic fever virus, Nat. Rev. Microbiol., 21, 463–477, https://doi.org/10.1038/s41579-023-00871-9, 2023.
Hulswit, R. J. G., Paesen, G. C., Bowden, T. A., and Shi, X.: Recent Advances in Bunyavirus Glycoprotein Research: Precursor Processing, Receptor Binding and Structure, Viruses, 13, 353, https://doi.org/10.3390/v13020353, 2021.
Jirasko, V., Lends, A., Lakomek, N.-A., Fogeron, M.-L., Weber, M., Malär, A., Penzel, S., Bartenschlager, R., Meier, B. H., and Böckmann, A.: Dimer organization of membrane-associated NS5A of hepatitis C virus as determined by highly sensitive 1H-detected solid-state NMR, Angewandte Chemie International Edition, 60, 5339–5347, https://doi.org/10.1002/anie.202013296, 2020.
Klukowski, P., Riek, R., and Güntert, P.: Rapid protein assignments and structures from raw NMR spectra with the deep learning technique ARTINA, Nat. Commun., 13, 6151, https://doi.org/10.1038/s41467-022-33879-5, 2022.
Laux, M. and Böckmann, A.: Crimean Congo Hemorrhagic Fever Gn zinc finger chemical shifts, BMRB accession number 52372 [data set], https://bmrb.io/data_library/summary/index.php?bmrbId=52372, 2024.
Lecoq, L., Brigandat, L., and Callon, M.: NMR data CCHFV Gn cytosolic domain solution spectra, Zenodo [data set], https://doi.org/10.5281/zenodo.10938432, 2024.
Lorenzo Juanes, H. M., Carbonell, C., Sendra, B. F., López-Bernus, A., Bahamonde, A., Orfao, A., Lista, C. V., Ledesma, M. S., Negredo, A. I., Rodríguez-Alonso, B., Bua, B. R., Sánchez-Seco, M. P., Muñoz Bellido, J. L., Muro, A., and Belhassen-García, M.: Crimean-Congo Hemorrhagic Fever, Spain, 2013–2021, Emerg. Infect. Dis., 29, 252–259, https://doi.org/10.3201/eid2902.220677, 2023.
Matsuda, T., Kigawa, T., Koshiba, S., Inoue, M., Aoki, M., Yamasaki, K., Seki, M., Shinozaki, K., and Yokoyama, S.: Cell-free synthesis of zinc-binding proteins, Journal of Structural and Functional Genomics, 7, 93–100, https://doi.org/10.1007/s10969-006-9012-1, 2006.
Mishra, A. K., Hellert, J., Freitas, N., Guardado-Calvo, P., Haouz, A., Fels, J. M., Maurer, D. P., Abelson, D. M., Bornholdt, Z. A., Walker, L. M., Chandran, K., Cosset, F.-L., McLellan, J. S., and Rey, F. A.: Structural basis of synergistic neutralization of Crimean-Congo hemorrhagic fever virus by human antibodies, Science, 375, 104–109, https://doi.org/10.1126/science.abl6502, 2022.
Shahhosseini, N., Wong, G., Babuadze, G., Camp, J. V., Ergonul, O., Kobinger, G. P., Chinikar, S., and Nowotny, N.: Crimean-Congo Hemorrhagic Fever Virus in Asia, Africa and Europe, Microorganisms, 9, 1907, https://doi.org/10.3390/microorganisms9091907, 2021.
Skinner, S. P., Fogh, R. H., Boucher, W., Ragan, T. J., Mureddu, L. G., and Vuister, G. W.: CcpNmr AnalysisAssign: a flexible platform for integrated NMR analysis, J. Biomol. NMR, 66, 111–124, https://doi.org/10.1007/s10858-016-0060-y, 2016.
Stevens, T. J., Fogh, R. H., Boucher, W., Higman, V. A., Eisenmenger, F., Bardiaux, B., Rossum, B.-J., Oschkinat, H., and Laue, E. D.: A software framework for analysing solid-state MAS NMR data, J. Biomol. NMR, 51, 437–447, https://doi.org/10.1007/s10858-011-9569-2, 2011.
Strandin, T., Hepojoki, J., and Vaheri, A.: Cytoplasmic tails of bunyavirus Gn glycoproteins–Could they act as matrix protein surrogates?, Virology, 437, 73–80, https://doi.org/10.1016/j.virol.2013.01.001, 2013.
Strecker, T., Eichler, R., Meulen, J. ter, Weissenhorn, W., Dieter Klenk, H., Garten, W., and Lenz, O.: Lassa Virus Z Protein Is a Matrix Protein Sufficient for the Release of Virus-Like Particles, J. Virol., 77, 10700–10705, https://doi.org/10.1128/JVI.77.19.10700-10705.2003, 2003.
Sun, Y., Li, J., Gao, G. F., Tien, P., and Liu, W.: Bunyavirales ribonucleoproteins: the viral replication and transcription machinery, Crit. Rev. Microbiol., 44, 522–540, https://doi.org/10.1080/1040841X.2018.1446901, 2018.
Takai, K., Sawasaki, T., and Endo, Y.: Practical cell-free protein synthesis system using purified wheat embryos, Nat. Protoc., 5, 227–238, https://doi.org/10.1038/nprot.2009.207, 2010.
Vranken, W. F., Boucher, W., Stevens, T. J., Fogh, R. H., Pajon, A., Llinas, M., Ulrich, E. L., Markley, J. L., Ionides, J., and Laue, E. D.: The CCPN data model for NMR spectroscopy: development of a software pipeline, Proteins: Structure, Function, and Bioinformatics, 59, 687–696, https://doi.org/10.1002/prot.20449, 2005.
Ye, W., Ye, C., Hu, Y., Dong, Y., Lei, Y., and Zhang, F.: The structure of Crimean-Congo hemorrhagic fever virus Gc is revealed; many more still need an answer, Virol. Sin., 37, 634–636, https://doi.org/10.1016/j.virs.2022.05.003, 2022.
Zhu, Y., Wu, Y., Chai, Y., Qi, J., Peng, R., Feng, W.-H., and Gao, G. F.: The Postfusion Structure of the Heartland Virus Gc Glycoprotein Supports Taxonomic Separation of the Bunyaviral Families Phenuiviridae and Hantaviridae, J. Virol., 92, e01558-17, https://doi.org/10.1128/JVI.01558-17, 2017.
Short summary
We used NMR to sequentially assign the side-chain resonances of the cytosolic domain of glycoprotein n of the Crimean–Congo hemorrhagic fever virus. The combination of cell-free protein synthesis with high-field NMR and artificial intelligence approaches facilitated a time- and effort-efficient approach. Our results will be harnessed to study the membrane-bound form of the domain and its interactions with virulence factors, which will ultimately help to understand their role in disease.
We used NMR to sequentially assign the side-chain resonances of the cytosolic domain of...