Articles | Volume 5, issue 2
https://doi.org/10.5194/mr-5-95-2024
https://doi.org/10.5194/mr-5-95-2024
Research article
 | 
08 Jul 2024
Research article |  | 08 Jul 2024

NMR side-chain assignments of the Crimean–Congo hemorrhagic fever virus glycoprotein n cytosolic domain

Louis Brigandat, Maëlys Laux, Caroline Marteau, Laura Cole, Anja Böckmann, Lauriane Lecoq, Marie-Laure Fogeron, and Morgane Callon

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Cited articles

Arragain, B., Reguera, J., Desfosses, A., Gutsche, I., Schoehn, G., and Malet, H.: High resolution cryo-EM structure of the helical RNA-bound Hantaan virus nucleocapsid reveals its assembly mechanisms, eLife, 8, e43075, https://doi.org/10.7554/eLife.43075, 2019. 
Arragain, B., Effantin, G., Gerlach, P., Reguera, J., Schoehn, G., Cusack, S., and Malet, H.: Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes, Nat. Commun., 11, 3590, https://doi.org/10.1038/s41467-020-17349-4, 2020. 
Arragain, B., Durieux Trouilleton, Q., Baudin, F., Provaznik, J., Azevedo, N., Cusack, S., Schoehn, G., and Malet, H.: Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription, Nat. Commun., 13, 902, https://doi.org/10.1038/s41467-022-28428-z, 2022. 
Bignon, E. A., Albornoz, A., Guardado-Calvo, P., Rey, F. A., and Tischler, N. D.: Molecular organization and dynamics of the fusion protein Gc at the hantavirus surface, eLife, 8, e46028, https://doi.org/10.7554/eLife.46028, 2019. 
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Short summary
We used NMR to sequentially assign the side-chain resonances of the cytosolic domain of glycoprotein n of the Crimean–Congo hemorrhagic fever virus. The combination of cell-free protein synthesis with high-field NMR and artificial intelligence approaches facilitated a time- and effort-efficient approach. Our results will be harnessed to study the membrane-bound form of the domain and its interactions with virulence factors, which will ultimately help to understand their role in disease.