Articles | Volume 6, issue 2
https://doi.org/10.5194/mr-6-281-2025
https://doi.org/10.5194/mr-6-281-2025
Research article
 | 
02 Dec 2025
Research article |  | 02 Dec 2025

Optimized shaped pulses for a 2D single-frequency technique for refocusing (SIFTER)

Paul A. S. Trenkler, Burkhard Endeward, Snorri T. Sigurdsson, and Thomas F. Prisner

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Cited articles

Abdullin, D., Rauh Corro, P., Hett, T., and Schiemann, O.: PDSFit: PDS data analysis in the presence of orientation selectivity, g-anisotropy, and exchange coupling, Magnetic Resonance in Chemistry, 62, 37–60, https://doi.org/10.1002/mrc.5415, 2024.  
Abé, C., Klose, D., Dietrich, F., Ziegler, W. H., Polyhach, Y., Jeschke, G., and Steinhoff, H.-J.: Orientation selective DEER measurements on vinculin tail at X-band frequencies reveal spin label orientations, Journal of Magnetic Resonance, 216, 53–61, https://doi.org/10.1016/j.jmr.2011.12.024, 2012. 
Ashuiev, A., Allouche, F., Wili, N., Searles, K., Klose, D., Copéret, C., and Jeschke, G.: Molecular and supported Ti(III)-alkyls: efficient ethylene polymerization driven by the π-character of metal–carbon bonds and back donation from a singly occupied molecular orbital, Chem. Sci., 12, 780–792, https://doi.org/10.1039/D0SC04436A, 2021. 
Baum, J., Tycko, R., and Pines, A.: Broadband and adiabatic inversion of a two-level system by phase-modulated pulses, Phys. Rev. A, 32, 3435–3447, https://doi.org/10.1103/PhysRevA.32.3435, 1985. 
Bohlen, J.-M., Rey, M., and Bodenhausen, G.: Refocusing with chirped pulses for broadband excitation without phase dispersion, Journal of Magnetic Resonance, 84, 191–197, https://doi.org/10.1016/0022-2364(89)90018-8, 1989. 
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Short summary
Pulsed electron paramagnetic resonance can measure distances and orientation between two paramagnetic markers. If they are rigidly attached to a biomolecule, advanced insights into the structure and dynamics of the biomolecule follow. We used chirp pulses to perform real two-dimensional experiments with much shorter experimental time compared to experiments with monochromatic microwave pulses. We also present new pulse sequences and give a detailed protocol for setting up such experiments.
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