Magnetic Resonance
Magnetic Resonance
MR
Articles | Volume 6, issue 1
Articles | Volume 6, issue 1
https://doi.org/10.5194/mr-6-93-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-6-93-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 6, issue 1
Research article
 | 
10 Mar 2025
Research article |  | 10 Mar 2025

ih-RIDME: a pulse EPR experiment to probe the heterogeneous nuclear environment

Sergei Kuzin, Victoriya N. Syryamina, Mian Qi, Moritz Fischer, Miriam Hülsmann, Adelheid Godt, Gunnar Jeschke, and Maxim Yulikov

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Latest update: 30 Oct 2025
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Short summary
A new pulse electron paramagnetic resonance method is presented to quantify the local nuclear environment of an electron spin centre. We provide a detailed assessment of this technique. We review the theoretical principles, discuss the data analysis and demonstrate an application to a spin-labelled macromolecule supported by in silico modelling. The method can be applied to the characterization of heterogeneous systems, such as unstructured or semi-structured (bio)macromolecules.
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