It is shown that proton long-lived states can be observed on benchtop nuclear magnetic resonance spectrometers in molecules containing short aliphatic chains. The effects of strong coupling between methylene groups can be mitigated by optimizing the amplitude and duration of the excitation pulse.

In achiral polyfluoroalkyl substances with two to three CF₂ groups, long-lived ¹⁹F spin states (T_LLS) were measured and found to last about three times longer than T₁ in a static 11.6 T field. These lifetimes are sensitive to macromolecular binding, making them useful for screening fluorinated drugs.

This tutorial paper shows how to simulate NMR spectra at zero to ultralow fields. The process is presented in detail, including the tricks that are usually omitted from research papers and assuming as little prior knowledge from the reader as possible. In this attempt to make NMR simulation approachable, the authors wish to pay tribute to the late Prof. Konstantin L’vovich Ivanov.

In this work, we study the relaxivity of long-lived states (LLSs) excited across two to three CH₂ groups in four different compounds and find that a commonly used polarising agent in dissolution dynamic nuclear polarization (d-DNP) does not enhance relaxation significantly. This result is important for the future implementation of d-DNP hyperpolarization of LLS.