Articles | Volume 7, issue 1
https://doi.org/10.5194/mr-7-29-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-7-29-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
16 Apr 2026
Research article |
| 16 Apr 2026
| 16 Apr 2026
Accelerated 19F biomolecular magic-angle spinning NMR with paramagnetic dopants
Lea M. Becker
Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria
Giorgia Toscano
Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria
Institute of Organic Chemistry, University of Vienna, Währinger Str. 38, 1090 Vienna, Austria
Anna Kapitonova
Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria
Rajkumar Singh
Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria
Undina Guillerm
Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria
Roman J. Lichtenecker
Institute of Organic Chemistry, University of Vienna, Währinger Str. 38, 1090 Vienna, Austria
Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria
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Short summary
Magic-angle-spinning nuclear magnetic resonance (NMR) is ideal for studying protein structure and dynamics. Introducing fluorine atoms offers advantages due to the NMR properties of 19F and the absence of natural fluorine. However, the slow spin polarisation recovery of 19F causes long recycle delays between scans. We demonstrate that adding paramagnetic co-solutes to solid protein samples largely accelerates these experiments, and we identify optimal conditions for this approach.
Magic-angle-spinning nuclear magnetic resonance (NMR) is ideal for studying protein structure...
