A protein was produced, where a single amino acid type was substituted globally by a fluorinated analogue, delivering multiple sites for interrogation by ¹⁹F-NMR spectroscopy. Substitution of methyl groups by CH₂F groups yields outstanding spectral resolution with minimal structural perturbation of the protein. Our work identifies the γ-gauche effect as the main reason for the spectral dispersion.

3D printed sample cells, inserted into standard 5-mm NMR tubes, are demonstrated to offer improved sensitivity for biomolecular NMR when the quantity of material is limiting. By using an ellipsoidal shape, magnetic field homogeneity inside the microcell to first order is insensitive to differences in the magnetic susceptibility of printer resin and solvent. The sample cells are particularly useful at high ionic strength and at ultra-high magnetic fields.

Parallel NMR detection enhances measurement throughput for high-throughput screening. However, local gradients in parallel detectors cause field spillover in adjacent channels, leading to spin dephasing and signal loss. This study introduces a compensation scheme using optimized pulses to mitigate gradient-induced field inhomogeneity through coherence locking. The proposed approach offers an effective solution for NMR probes with parallel, independently switchable gradient coils.

Nuclear quadrupole resonance is a sensitive technique for studying local structure. However, materials whose local structure is disordered can produce very wide spectra that are both technically challenging and require a lot of human-hours to measure. We present a setup that acquires these spectra automatically over a wide frequency range. We demonstrate this setup on several lead halide perovskites at variable temperatures and discuss the details uncovered by high resolution spectra.

Investigating NMR phenomena at variable magnetic fields is useful and insightful, in particular for hyperpolarization and molecular dynamics. To benefit from high-resolution at high magnetic fields, field-dependent investigations can be performed on a high-field NMR spectrometer, using a sample shuttle apparatus for field cycling. Here we introduce a new design of sample shuttle, which is fast, reliable, and narrow. We show a series of applications to small molecules and a protein in solution.

Flavoproteins are essential biomolecules involved in many vital processes. Understanding their reactivity requires studying their intermediate semiquinone form. Using advanced spectroscopy and isotopic labeling, we measured key interactions within this state, uncovering subtle changes in the distribution of electron density. These findings refine our understanding of flavoprotein function and highlight the value of combining experiments and computations for studying complex biological systems.

We present a technology that allows the direct writing of conductive tracks on cylindrical substrates as receiver coils for magnetic resonance experiments. The structures are written with high precision, which has two benefits. First the real structures behave pretty similar to the simulated designs, second it allows the writing of coils others then the fairly straight forward solenoidal coils, thereby making other designs available for MR micro-coils.