Flavoproteins are essential biomolecules involved in many vital processes. Understanding their reactivity requires studying their intermediate semiquinone form. Using advanced spectroscopy and isotopic labeling, we measured key interactions within this state, uncovering subtle changes in the distribution of electron density. These findings refine our understanding of flavoprotein function and highlight the value of combining experiments and computations for studying complex biological systems.

We present a technology that allows the direct writing of conductive tracks on cylindrical substrates as receiver coils for magnetic resonance experiments. The structures are written with high precision, which has two benefits. First the real structures behave pretty similar to the simulated designs, second it allows the writing of coils others then the fairly straight forward solenoidal coils, thereby making other designs available for MR micro-coils.

Local dynamics in rotating solids can be investigated by measuring enhanced transverse relaxation rates near rotary resonance conditions. In contrast, this effect is not expected in rotating solutions, as first-order anisotropic interactions are averaged out. For rotating solutions, we show how enhanced transverse relaxation near rotary resonance reports on fluctuations that are introduced due to imperfect hardware, with rf-field imperfections being the primary source of the effect.

We have performed a low frequency EPR study on two solid-state batteries (NMC||Li-metal and NMC||Si-based) sealed inside an industrial and standard aluminum laminated pouch cell. EPR imaging allowed us to locate the metallic anode through the Al-based pouch cell confirming the good penetration of the microwave field through the pouch. We think our approach provides valuable insight for the battery community paving the way to the quality control of industrial and commercial batteries.

Quantification of heterogeneous systems such as unstructured or semi-structured (bio)macromolecules is an important but challenging task. Pulse EPR methods can contribute by characterizing the local nuclear environment of a spin centre. Here, we provide a detailed assessment of a pulse EPR technique derived from a RIDME experiment. We review the theoretical principles, discuss the data analysis and demonstrate an application to a spin-labeled macromolecule supported by in silico modelling.

The time evolution of a spin system in NMR can be represented in simple cases by an analytical equation, which is often better suited to promote physical understanding for teaching as well as for scientific discussion than numerical results. A method is presented that can be used to find analytical equations even for moderately complex problems. This is based on the fact that the coefficients of suitable commutator equation systems can be inserted directly into templates for propagation rules.