Analysis of the electronic structure of the primary electron donor of photosystem I of <i>Spirodela</i> <i>oligorrhiza</i> by photochemically induced dynamic nuclear polarization (photo-CIDNP) solid-state nuclear  magnetic  resonance (NMR)

Janssen, Geertje J.; Eschenbach, Patrick; Kurle, Patrick; Bode, Bela E.; Neugebauer, Johannes; de Groot, Huub J. M.; Matysik, Jörg; Alia, Alia

doi:https://doi.org/10.5194/mr-1-261-2020

Articles | Volume 1, issue 2

https://doi.org/10.5194/mr-1-261-2020

© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Special issue:

Robert Kaptein Festschrift

https://doi.org/10.5194/mr-1-261-2020

© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 1, issue 2

Research article

|

13 Nov 2020

Research article |

| 13 Nov 2020

Analysis of the electronic structure of the primary electron donor of photosystem I of Spirodela oligorrhiza by photochemically induced dynamic nuclear polarization (photo-CIDNP) solid-state nuclear magnetic resonance (NMR)

Geertje J. Janssen, Patrick Eschenbach, Patrick Kurle, Bela E. Bode, Johannes Neugebauer, Huub J. M. de Groot, Jörg Matysik, and Alia Alia

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Final revised paper (published on 13 Nov 2020)
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Preprint (discussion started on 28 Sep 2020)
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Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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RC1: 'Analysis of the electronic asymmetry of the primary electron donor of photosystem I of Spirodela oligorrhiza by photo-CIDNP solid-state NMR', Peter Hore, 02 Oct 2020
AC1: 'Point-by-point reply', Jörg Matysik, 09 Oct 2020
- RC3: 'Authors' response to my comments', Peter Hore, 16 Oct 2020
RC2: 'Functional symmetry of electron transfer despite chemical-shift asymmetry', Gunnar Jeschke, 12 Oct 2020
- AC2: 'Reply to reviewer Gunnar Jeschke', Jörg Matysik, 16 Oct 2020

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

AR by Jörg Matysik on behalf of the Authors (27 Oct 2020) Author's response Manuscript

ED: Publish subject to corrections (28 Oct 2020) by Rolf Boelens

AR by Jörg Matysik on behalf of the Authors (29 Oct 2020) Author's response Manuscript

Short summary

Natural photosynthetic reaction centers (RCs) are built up by two parallel branches of cofactors. While photosystem II and purple bacterial RCs selectively use one branch for light-driven electron transfer, photosystem I, as also shown here, is using both branches. Comparing NMR chemical shifts, we shown that the two donor cofactors in photosystem I are similarly distinguished to those in purple bacterial RCs (Schulten et al., 2002; Biochemistry 41, 8708). Alternative reasons are discussed.