Articles | Volume 1, issue 2
Magn. Reson., 1, 285–299, 2020
https://doi.org/10.5194/mr-1-285-2020
Magn. Reson., 1, 285–299, 2020
https://doi.org/10.5194/mr-1-285-2020

Research article 09 Dec 2020

Research article | 09 Dec 2020

Strategies to identify and suppress crosstalk signals in double electron–electron resonance (DEER) experiments with gadoliniumIII and nitroxide spin-labeled compounds

Markus Teucher et al.

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Interactive discussion

Status: closed
Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
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Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision
AR by Enrica Bordignon on behalf of the Authors (03 Sep 2020)  Author's response    Manuscript
ED: Referee Nomination & Report Request started (21 Sep 2020) by Thomas Prisner
RR by Anonymous Referee #2 (06 Oct 2020)
RR by Anonymous Referee #1 (09 Oct 2020)
ED: Publish subject to minor revisions (review by editor) (15 Oct 2020) by Thomas Prisner
AR by Enrica Bordignon on behalf of the Authors (26 Oct 2020)  Author's response    Manuscript
ED: Publish as is (31 Oct 2020) by Thomas Prisner
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Short summary
With a pulsed dipolar electron paramagnetic resonance technique named double electron–electron resonance (DEER), we measure nanometer distances between spin labels attached to biomolecules. If more than one spin type is present (A and B), we can separately address AA, AB, and BB distances via distinct spectroscopic channels, increasing the information content per sample. Here, we investigate the appearance of unwanted channel crosstalks in DEER and suggest ways to identify and suppress them.