Magnetic Resonance
Magnetic Resonance
MR
Articles | Volume 1, issue 2
Articles | Volume 1, issue 2
https://doi.org/10.5194/mr-1-285-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-1-285-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 1, issue 2
Research article
 | 
09 Dec 2020
Research article |  | 09 Dec 2020

Strategies to identify and suppress crosstalk signals in double electron–electron resonance (DEER) experiments with gadoliniumIII and nitroxide spin-labeled compounds

Markus Teucher, Mian Qi, Ninive Cati, Henrik Hintz, Adelheid Godt, and Enrica Bordignon

Related authors

ih-RIDME: a pulse EPR experiment to probe the heterogeneous nuclear environment
Sergei Kuzin, Victoriya N. Syryamina, Mian Qi, Moritz Fischer, Miriam Hülsmann, Adelheid Godt, Gunnar Jeschke, and Maxim Yulikov
Magn. Reson. Discuss., https://doi.org/10.5194/mr-2024-19,https://doi.org/10.5194/mr-2024-19, 2024
Preprint under review for MR
Short summary
Electron-spin decoherence in trityl radicals in the absence and presence of microwave irradiation
Gunnar Jeschke, Nino Wili, Yufei Wu, Sergei Kuzin, Hugo Karas, Henrik Hintz, and Adelheid Godt
Magn. Reson. Discuss., https://doi.org/10.5194/mr-2024-17,https://doi.org/10.5194/mr-2024-17, 2024
Revised manuscript accepted for MR
Short summary
Intermolecular contributions, filtration effects and signal composition of SIFTER (single-frequency technique for refocusing)
Agathe Vanas, Janne Soetbeer, Frauke Diana Breitgoff, Henrik Hintz, Muhammad Sajid, Yevhen Polyhach, Adelheid Godt, Gunnar Jeschke, Maxim Yulikov, and Daniel Klose
Magn. Reson., 4, 1–18, https://doi.org/10.5194/mr-4-1-2023,https://doi.org/10.5194/mr-4-1-2023, 2023
Short summary
High-sensitivity Gd3+–Gd3+ EPR distance measurements that eliminate artefacts seen at short distances
Hassane EL Mkami, Robert I. Hunter, Paul A. S. Cruickshank, Michael J. Taylor, Janet E. Lovett, Akiva Feintuch, Mian Qi, Adelheid Godt, and Graham M. Smith
Magn. Reson., 1, 301–313, https://doi.org/10.5194/mr-1-301-2020,https://doi.org/10.5194/mr-1-301-2020, 2020
Short summary
Distance measurement between trityl radicals by pulse dressed electron paramagnetic resonance with phase modulation
Nino Wili, Henrik Hintz, Agathe Vanas, Adelheid Godt, and Gunnar Jeschke
Magn. Reson., 1, 75–87, https://doi.org/10.5194/mr-1-75-2020,https://doi.org/10.5194/mr-1-75-2020, 2020
Short summary
More articles (1)

Related subject area

Field: EPR | Topic: Pulse-sequence development
Increased sensitivity in Electron Nuclear Double Resonance spectroscopy with chirped radiofrequency pulses
Julian Stropp, Nino Wili, Niels Christian Nielsen, and Daniel Klose
Magn. Reson. Discuss., https://doi.org/10.5194/mr-2024-14,https://doi.org/10.5194/mr-2024-14, 2024
Revised manuscript accepted for MR
Short summary
The decay of the refocused Hahn echo in double electron–electron resonance (DEER) experiments
Thorsten Bahrenberg, Samuel M. Jahn, Akiva Feintuch, Stefan Stoll, and Daniella Goldfarb
Magn. Reson., 2, 161–173, https://doi.org/10.5194/mr-2-161-2021,https://doi.org/10.5194/mr-2-161-2021, 2021
Short summary
Distance measurement between trityl radicals by pulse dressed electron paramagnetic resonance with phase modulation
Nino Wili, Henrik Hintz, Agathe Vanas, Adelheid Godt, and Gunnar Jeschke
Magn. Reson., 1, 75–87, https://doi.org/10.5194/mr-1-75-2020,https://doi.org/10.5194/mr-1-75-2020, 2020
Short summary
Optimising broadband pulses for DEER depends on concentration and distance range of interest
Andreas Scherer, Sonja Tischlik, Sabrina Weickert, Valentin Wittmann, and Malte Drescher
Magn. Reson., 1, 59–74, https://doi.org/10.5194/mr-1-59-2020,https://doi.org/10.5194/mr-1-59-2020, 2020
Short summary

Cited articles

Abdullin, D., Duthie, F., Meyer, A., Müller, E. S., Hagelueken, G., and Schiemann, O.: Comparison of PELDOR and RIDME for distance measurements between nitroxides and low-spin Fe (III) ions, J. Phys. Chem. B, 119, 13534–13542, 2015. a
Ackermann, K., Pliotas, C., Valera, S., Naismith, J. H., and Bode, B. E.: Sparse labeling PELDOR spectroscopy on multimeric mechanosensitive membrane channels, Biophys. J., 113, 1968–1978, 2017. a
Akhmetzyanov, D., Plackmeyer, J., Endeward, B., Denysenkov, V., and Prisner, T.: Pulsed electron-electron double resonance spectroscopy between a high-spin Mn2+ ion and a nitroxide spin label, Phys. Chem. Chem. Phys., 17, 6760–6766, 2015. a
Altenbach, C.: LongDistances, availabe at: http://www.biochemistry.ucla.edu/Faculty/Hubbell/, last access: 31 August 2020. a
Bahrenberg, T., Rosenski, Y., Carmieli, R., Zibzener, K., Qi, M., Frydman, V., Godt, A., Goldfarb, D., and Feintuch, A.: Improved sensitivity for W-band Gd (III)-Gd (III) and nitroxide-nitroxide DEER measurements with shaped pulses, J. Magn. Reson., 283, 1–13, 2017. a
More articles (61)
Download
Short summary
With a pulsed dipolar electron paramagnetic resonance technique named double electron–electron resonance (DEER), we measure nanometer distances between spin labels attached to biomolecules. If more than one spin type is present (A and B), we can separately address AA, AB, and BB distances via distinct spectroscopic channels, increasing the information content per sample. Here, we investigate the appearance of unwanted channel crosstalks in DEER and suggest ways to identify and suppress them.
Read more