Articles | Volume 1, issue 2
Magn. Reson., 1, 285–299, 2020
https://doi.org/10.5194/mr-1-285-2020
Magn. Reson., 1, 285–299, 2020
https://doi.org/10.5194/mr-1-285-2020

Research article 09 Dec 2020

Research article | 09 Dec 2020

Strategies to identify and suppress crosstalk signals in double electron–electron resonance (DEER) experiments with gadoliniumIII and nitroxide spin-labeled compounds

Markus Teucher et al.

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Cited articles

Abdullin, D., Duthie, F., Meyer, A., Müller, E. S., Hagelueken, G., and Schiemann, O.: Comparison of PELDOR and RIDME for distance measurements between nitroxides and low-spin Fe (III) ions, J. Phys. Chem. B, 119, 13534–13542, 2015. a
Ackermann, K., Pliotas, C., Valera, S., Naismith, J. H., and Bode, B. E.: Sparse labeling PELDOR spectroscopy on multimeric mechanosensitive membrane channels, Biophys. J., 113, 1968–1978, 2017. a
Akhmetzyanov, D., Plackmeyer, J., Endeward, B., Denysenkov, V., and Prisner, T.: Pulsed electron-electron double resonance spectroscopy between a high-spin Mn2+ ion and a nitroxide spin label, Phys. Chem. Chem. Phys., 17, 6760–6766, 2015. a
Altenbach, C.: LongDistances, availabe at: http://www.biochemistry.ucla.edu/Faculty/Hubbell/, last access: 31 August 2020. a
Bahrenberg, T., Rosenski, Y., Carmieli, R., Zibzener, K., Qi, M., Frydman, V., Godt, A., Goldfarb, D., and Feintuch, A.: Improved sensitivity for W-band Gd (III)-Gd (III) and nitroxide-nitroxide DEER measurements with shaped pulses, J. Magn. Reson., 283, 1–13, 2017. a
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Short summary
With a pulsed dipolar electron paramagnetic resonance technique named double electron–electron resonance (DEER), we measure nanometer distances between spin labels attached to biomolecules. If more than one spin type is present (A and B), we can separately address AA, AB, and BB distances via distinct spectroscopic channels, increasing the information content per sample. Here, we investigate the appearance of unwanted channel crosstalks in DEER and suggest ways to identify and suppress them.