Magnetic Resonance
Magnetic Resonance
MR
Articles | Volume 1, issue 2
Articles | Volume 1, issue 2
https://doi.org/10.5194/mr-1-285-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-1-285-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 1, issue 2
Research article
 | 
09 Dec 2020
Research article |  | 09 Dec 2020

Strategies to identify and suppress crosstalk signals in double electron–electron resonance (DEER) experiments with gadoliniumIII and nitroxide spin-labeled compounds

Markus Teucher, Mian Qi, Ninive Cati, Henrik Hintz, Adelheid Godt, and Enrica Bordignon

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Short summary
With a pulsed dipolar electron paramagnetic resonance technique named double electron–electron resonance (DEER), we measure nanometer distances between spin labels attached to biomolecules. If more than one spin type is present (A and B), we can separately address AA, AB, and BB distances via distinct spectroscopic channels, increasing the information content per sample. Here, we investigate the appearance of unwanted channel crosstalks in DEER and suggest ways to identify and suppress them.
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