Magnetic Resonance
Magnetic Resonance
Magnetic Resonance
Articles | Volume 1, issue 2
Articles | Volume 1, issue 2
Magn. Reson., 1, 301–313, 2020
https://doi.org/10.5194/mr-1-301-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
Magn. Reson., 1, 301–313, 2020
https://doi.org/10.5194/mr-1-301-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 1, issue 2

Research article 09 Dec 2020

Research article | 09 Dec 2020

High-sensitivity Gd3+–Gd3+ EPR distance measurements that eliminate artefacts seen at short distances

Hassane EL Mkami et al.

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Short summary
Through a series of DEER measurements on two Gd rulers, with Gd–Gd distances of 2.1 and 6.0 nm, we show that artefacts commonly observed when measuring short distances can be eliminated by avoiding excitation of the central transition by both the pump and observer pulses. By using a wideband induction mode sample holder at 94 GHz, we demonstrate that high-quality DEER measurements will become possible using Gd spin labels at sub-µM concentrations, with implications for in-cell DEER measurements.
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