Articles | Volume 1, issue 2
Magn. Reson., 1, 301–313, 2020
Magn. Reson., 1, 301–313, 2020

Research article 09 Dec 2020

Research article | 09 Dec 2020

High-sensitivity Gd3+–Gd3+ EPR distance measurements that eliminate artefacts seen at short distances

Hassane EL Mkami et al.


Interactive discussion

Status: closed
Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
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Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision
AR by Hassane EL Mkami on behalf of the Authors (13 Nov 2020)  Author's response    Manuscript
ED: Publish as is (18 Nov 2020) by Thomas Prisner
Short summary
Through a series of DEER measurements on two Gd rulers, with Gd–Gd distances of 2.1 and 6.0 nm, we show that artefacts commonly observed when measuring short distances can be eliminated by avoiding excitation of the central transition by both the pump and observer pulses. By using a wideband induction mode sample holder at 94 GHz, we demonstrate that high-quality DEER measurements will become possible using Gd spin labels at sub-µM concentrations, with implications for in-cell DEER measurements.