The decay of the refocused Hahn echo in double electron–electron resonance (DEER) experiments

Bahrenberg, Thorsten; Jahn, Samuel M.; Feintuch, Akiva; Stoll, Stefan; Goldfarb, Daniella

doi:https://doi.org/10.5194/mr-2-161-2021

Articles | Volume 2, issue 1

https://doi.org/10.5194/mr-2-161-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

https://doi.org/10.5194/mr-2-161-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 2, issue 1

Research article

|

16 Apr 2021

Research article |

| 16 Apr 2021

The decay of the refocused Hahn echo in double electron–electron resonance (DEER) experiments

Thorsten Bahrenberg, Samuel M. Jahn, Akiva Feintuch, Stefan Stoll, and Daniella Goldfarb

Supplement

https://doi.org/10.5194/mr-2-161-2021-supplement

Data sets

The decay of the refocused Hahn echo in DEER experiments Thorsten Bahrenberg, Samuel M. Jahn, Akiva Feintuch, Stefan Stoll, and Daniella Goldfarb https://doi.org/10.5281/zenodo.4449018

Short summary

Double electron–electron resonance (DEER) provides information on the structure of proteins by attaching two spin labels to the protein at a well-defined location and measuring the distance between them. The sensitivity of the method in terms of the amount of the protein that is needed for the experiment depends strongly on the relaxation properties of the spin label and the composition of the solvent. We show how to set up the experiment for best sensitivity when the solvent is water (H₂O).