Articles | Volume 2, issue 1
https://doi.org/10.5194/mr-2-161-2021
https://doi.org/10.5194/mr-2-161-2021
Research article
 | 
16 Apr 2021
Research article |  | 16 Apr 2021

The decay of the refocused Hahn echo in double electron–electron resonance (DEER) experiments

Thorsten Bahrenberg, Samuel M. Jahn, Akiva Feintuch, Stefan Stoll, and Daniella Goldfarb

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Latest update: 27 Mar 2024
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Short summary
Double electron–electron resonance (DEER) provides information on the structure of proteins by attaching two spin labels to the protein at a well-defined location and measuring the distance between them. The sensitivity of the method in terms of the amount of the protein that is needed for the experiment depends strongly on the relaxation properties of the spin label and the composition of the solvent. We show how to set up the experiment for best sensitivity when the solvent is water (H2O).