Articles | Volume 2, issue 1
Research article
27 Apr 2021
Research article |  | 27 Apr 2021

Competing transfer pathways in direct and indirect dynamic nuclear polarization magic anglespinning nuclear magnetic resonance experiments on HIV-1 capsid assemblies: implications for sensitivity and resolution

Ivan V. Sergeyev, Caitlin M. Quinn, Jochem Struppe, Angela M. Gronenborn, and Tatyana Polenova


Interactive discussion

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2021-13', Anja Böckmann, 05 Feb 2021
    • AC1: 'Reply on RC1', Tatyana Polenova, 23 Mar 2021
  • RC2: 'Comment on mr-2021-13', Björn Corzilius, 23 Feb 2021
  • RC3: 'Comment on mr-2021-13', Anonymous Referee #3, 02 Mar 2021
    • AC3: 'Reply on RC3', Tatyana Polenova, 23 Mar 2021

Peer review completion

AR: Author's response | RR: Referee report | ED: Editor decision
AR by Tatyana Polenova on behalf of the Authors (23 Mar 2021)  Author's response    Author's tracked changes    Manuscript
ED: Publish as is (23 Mar 2021) by Rolf Boelens
Short summary
Dynamic nuclear polarization (DNP) magic angle spinning NMR yields huge signal enhancements and is attractive for signal-limited cases. We studied polarization transfer pathways underlying sensitivity and resolution in DNP experiments on tubular assemblies of HIV-1 CA protein. All three polarization transfer pathways are simultaneously active and can be selected by carefully choosing experimental conditions. Our study highlights the unique capabilities of DNP for structural biology applications.