Articles | Volume 2, issue 1
Magn. Reson., 2, 239–249, 2021
https://doi.org/10.5194/mr-2-239-2021

Special issue: Robert Kaptein Festschrift

Magn. Reson., 2, 239–249, 2021
https://doi.org/10.5194/mr-2-239-2021

Research article 27 Apr 2021

Research article | 27 Apr 2021

Competing transfer pathways in direct and indirect dynamic nuclear polarization magic anglespinning nuclear magnetic resonance experiments on HIV-1 capsid assemblies: implications for sensitivity and resolution

Ivan V. Sergeyev et al.

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Magn. Reson. Discuss., https://doi.org/10.5194/mr-2021-53,https://doi.org/10.5194/mr-2021-53, 2021
Revised manuscript under review for MR
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Cited articles

Akbey, Ü., Franks, W. T., Linden, A., Lange, S., Griffin, R. G., van Rossum, B.-J., and Oschkinat, H.: Dynamic Nuclear Polarization of Deuterated Proteins, Angew. Chem. Int. Ed. Engl., 49, 7803–7806, https://doi.org/10.1002/anie.201002044, 2010. 
Aladin, V. and Corzilius, B.: Methyl Dynamics in Amino Acids Modulate Heteronuclear Cross Relaxation in the Solid State Under MAS DNP, Solid State Nucl. Mag., 99, 27–35, https://doi.org/10.1016/j.ssnmr.2019.02.004, 2019. 
Aladin, V. and Corzilius, B.: Direct Dynamic Nuclear Polarization as a Tool or Selective NMR Spectroscopy of Biomolecules, eMagRes, 9, 239–249, https://doi.org/10.1002/9780470034590.emrstm1597, 2020. 
Albert, B. J., Gao, C., Sesti, E. L., Saliba, E. P., Alaniva, N., Scott, F. J., Sigurdsson, S. T., and Barnes, A. B.: Dynamic Nuclear Polarization Nuclear Magnetic Resonance in Human Cells Using Fluorescent Polarizing Agents, Biochemistry, 57, 4741–4746, https://doi.org/10.1021/acs.biochem.8b00257, 2018. 
Bajaj, V. S., Farrar, C. T., Hornstein, M. K., Mastovsky, I., Vieregg, J., Bryant, J., Elena, B., Kreischer, K. E., Temkin, R. J., and Griffin, R. G.: Dynamic Nuclear Polarization at 9 T Using a Novel 250 GHz Gyrotron Microwave Source, J. Magn. Reson., 160, 85–90, https://doi.org/10.1016/S1090-7807(02)00192-1, 2003. 
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Short summary
Dynamic nuclear polarization (DNP) magic angle spinning NMR yields huge signal enhancements and is attractive for signal-limited cases. We studied polarization transfer pathways underlying sensitivity and resolution in DNP experiments on tubular assemblies of HIV-1 CA protein. All three polarization transfer pathways are simultaneously active and can be selected by carefully choosing experimental conditions. Our study highlights the unique capabilities of DNP for structural biology applications.