Articles | Volume 2, issue 1
Research article
27 Apr 2021
Research article |  | 27 Apr 2021

Competing transfer pathways in direct and indirect dynamic nuclear polarization magic anglespinning nuclear magnetic resonance experiments on HIV-1 capsid assemblies: implications for sensitivity and resolution

Ivan V. Sergeyev, Caitlin M. Quinn, Jochem Struppe, Angela M. Gronenborn, and Tatyana Polenova

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Short summary

Cited articles

Akbey, Ü., Franks, W. T., Linden, A., Lange, S., Griffin, R. G., van Rossum, B.-J., and Oschkinat, H.: Dynamic Nuclear Polarization of Deuterated Proteins, Angew. Chem. Int. Ed. Engl., 49, 7803–7806,, 2010. 
Aladin, V. and Corzilius, B.: Methyl Dynamics in Amino Acids Modulate Heteronuclear Cross Relaxation in the Solid State Under MAS DNP, Solid State Nucl. Mag., 99, 27–35,, 2019. 
Aladin, V. and Corzilius, B.: Direct Dynamic Nuclear Polarization as a Tool or Selective NMR Spectroscopy of Biomolecules, eMagRes, 9, 239–249,, 2020. 
Albert, B. J., Gao, C., Sesti, E. L., Saliba, E. P., Alaniva, N., Scott, F. J., Sigurdsson, S. T., and Barnes, A. B.: Dynamic Nuclear Polarization Nuclear Magnetic Resonance in Human Cells Using Fluorescent Polarizing Agents, Biochemistry, 57, 4741–4746,, 2018. 
Bajaj, V. S., Farrar, C. T., Hornstein, M. K., Mastovsky, I., Vieregg, J., Bryant, J., Elena, B., Kreischer, K. E., Temkin, R. J., and Griffin, R. G.: Dynamic Nuclear Polarization at 9 T Using a Novel 250 GHz Gyrotron Microwave Source, J. Magn. Reson., 160, 85–90,, 2003. 
Short summary
Dynamic nuclear polarization (DNP) magic angle spinning NMR yields huge signal enhancements and is attractive for signal-limited cases. We studied polarization transfer pathways underlying sensitivity and resolution in DNP experiments on tubular assemblies of HIV-1 CA protein. All three polarization transfer pathways are simultaneously active and can be selected by carefully choosing experimental conditions. Our study highlights the unique capabilities of DNP for structural biology applications.