Articles | Volume 2, issue 1
Magn. Reson., 2, 239–249, 2021
https://doi.org/10.5194/mr-2-239-2021

Special issue: Robert Kaptein Festschrift

Magn. Reson., 2, 239–249, 2021
https://doi.org/10.5194/mr-2-239-2021

Research article 27 Apr 2021

Research article | 27 Apr 2021

Competing transfer pathways in direct and indirect dynamic nuclear polarization magic anglespinning nuclear magnetic resonance experiments on HIV-1 capsid assemblies: implications for sensitivity and resolution

Ivan V. Sergeyev et al.

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Short summary
Dynamic nuclear polarization (DNP) magic angle spinning NMR yields huge signal enhancements and is attractive for signal-limited cases. We studied polarization transfer pathways underlying sensitivity and resolution in DNP experiments on tubular assemblies of HIV-1 CA protein. All three polarization transfer pathways are simultaneously active and can be selected by carefully choosing experimental conditions. Our study highlights the unique capabilities of DNP for structural biology applications.