Competing transfer pathways in direct and indirect dynamic nuclear polarization magic anglespinning nuclear magnetic resonance experiments on HIV-1 capsid assemblies: implications for sensitivity and resolution
Ivan V. Sergeyev,Caitlin M. Quinn,Jochem Struppe,Angela M. Gronenborn,and Tatyana Polenova
Ivan V. Sergeyev
Bruker Biospin Corporation, 15 Fortune Drive, Billerica, MA 01821, United States
Caitlin M. Quinn
Department of Chemistry and Biochemistry, University of Delaware,
Newark, DE 19716, United States
Pittsburgh Center for HIV Protein Interactions, University of
Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth
Avenue, Pittsburgh, PA 15261, United States
Department of Structural Biology, University of Pittsburgh School of Medicine, 3501 Fifth Ave., Pittsburgh, PA 15261, United States
Department of Chemistry and Biochemistry, University of Delaware,
Newark, DE 19716, United States
Pittsburgh Center for HIV Protein Interactions, University of
Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth
Avenue, Pittsburgh, PA 15261, United States
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Dynamic nuclear polarization (DNP) magic angle spinning NMR yields huge signal enhancements and is attractive for signal-limited cases. We studied polarization transfer pathways underlying sensitivity and resolution in DNP experiments on tubular assemblies of HIV-1 CA protein. All three polarization transfer pathways are simultaneously active and can be selected by carefully choosing experimental conditions. Our study highlights the unique capabilities of DNP for structural biology applications.
