Competing transfer pathways in direct and indirect dynamic nuclear polarization magic anglespinning nuclear magnetic resonance experiments on HIV-1 capsid assemblies: implications for sensitivity and resolution
Ivan V. Sergeyev,Caitlin M. Quinn,Jochem Struppe,Angela M. Gronenborn,and Tatyana Polenova
Ivan V. Sergeyev
Bruker Biospin Corporation, 15 Fortune Drive, Billerica, MA 01821, United States
Caitlin M. Quinn
Department of Chemistry and Biochemistry, University of Delaware,
Newark, DE 19716, United States
Pittsburgh Center for HIV Protein Interactions, University of
Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth
Avenue, Pittsburgh, PA 15261, United States
Department of Structural Biology, University of Pittsburgh School of Medicine, 3501 Fifth Ave., Pittsburgh, PA 15261, United States
Department of Chemistry and Biochemistry, University of Delaware,
Newark, DE 19716, United States
Pittsburgh Center for HIV Protein Interactions, University of
Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth
Avenue, Pittsburgh, PA 15261, United States
Viewed
Total article views: 1,589 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
998
547
44
1,589
199
23
24
HTML: 998
PDF: 547
XML: 44
Total: 1,589
Supplement: 199
BibTeX: 23
EndNote: 24
Views and downloads (calculated since 04 Feb 2021)
Cumulative views and downloads
(calculated since 04 Feb 2021)
Total article views: 1,060 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
652
378
30
1,060
116
22
23
HTML: 652
PDF: 378
XML: 30
Total: 1,060
Supplement: 116
BibTeX: 22
EndNote: 23
Views and downloads (calculated since 27 Apr 2021)
Cumulative views and downloads
(calculated since 27 Apr 2021)
Total article views: 529 (including HTML, PDF, and XML)
HTML
PDF
XML
Total
Supplement
BibTeX
EndNote
346
169
14
529
83
1
1
HTML: 346
PDF: 169
XML: 14
Total: 529
Supplement: 83
BibTeX: 1
EndNote: 1
Views and downloads (calculated since 04 Feb 2021)
Cumulative views and downloads
(calculated since 04 Feb 2021)
Viewed (geographical distribution)
Total article views: 1,589 (including HTML, PDF, and XML)
Thereof 1,382 with geography defined
and 207 with unknown origin.
Total article views: 1,060 (including HTML, PDF, and XML)
Thereof 950 with geography defined
and 110 with unknown origin.
Total article views: 529 (including HTML, PDF, and XML)
Thereof 432 with geography defined
and 97 with unknown origin.
Dynamic nuclear polarization (DNP) magic angle spinning NMR yields huge signal enhancements and is attractive for signal-limited cases. We studied polarization transfer pathways underlying sensitivity and resolution in DNP experiments on tubular assemblies of HIV-1 CA protein. All three polarization transfer pathways are simultaneously active and can be selected by carefully choosing experimental conditions. Our study highlights the unique capabilities of DNP for structural biology applications.
