Open-source, partially 3D-printed, high-pressure (50-bar) liquid-nitrogen-cooled parahydrogen generator
- Section for Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, Kiel 24118, Germany
Correspondence: Frowin Ellermann (email@example.com), and Jan-Bernd Hövener (firstname.lastname@example.org)
The signal of magnetic resonance imaging (MRI) can be enhanced by several orders of magnitude using hyperpolarization. In comparison to a broadly used dynamic nuclear polarization (DNP) technique that is already used in clinical trials, the parahydrogen (pH2)-based hyperpolarization approaches are less cost-intensive, are scalable, and offer high throughput. However, a pH2 generator is necessary. Available commercial pH2 generators are relatively expensive (EUR 10 000–150 000). To facilitate the spread of pH2-based hyperpolarization studies, here we provide the blueprints and 3D models as open-source for a low-cost (EUR <3000) 50-bar liquid-nitrogen-cooled pH2 generator.
Nuclear magnetic resonance (NMR) as well as magnetic resonance imaging (MRI) are widely used in medical imaging and chemical analysis. Despite the great success of these techniques (Feyter et al., 2018; Lange et al., 2008; Watson et al., 2020), the low signal-to-noise ratio of NMR limits promising applications such as in vivo spectroscopy or imaging of nuclei other than 1H (Wilferth et al., 2020; Xu et al., 2008). The hyperpolarization of nuclear spins boosts the signal of selected molecules by orders of magnitude. This way, imaging of the lung or metabolism has become feasible (Beek et al., 2004; Kurhanewicz et al., 2011).
Among techniques, parahydrogen and synthesis allows dramatically enhanced nuclear alignment (PASADENA) (Bowers and Weitekamp, 1986, 1987; Eisenschmid et al., 1987) has found application from catalysis research to metabolic imaging (Hövener et al., 2018; Kovtunov et al., 2018).
The production of parahydrogen (pH2) is relatively easy: H2 gas flows through a catalyst at cold temperatures; maximum para-enrichment of almost 100 % is achieved at about 25 K (Gamliel et al., 2010; Jeong et al., 2018; Kiryutin et al., 2017). To reach low temperatures, hence enriched pH2, liquid cryogens (Buckenmaier et al., 2018; Jeong et al., 2018) or electric cryopumps (Feng et al., 2012) are used. Electronic setups were reported, e.g. for pressures up to 50 bar of ≈100 % pH2 (Hövener et al., 2013). Liquid nitrogen (lN2)-based systems were presented, however often with limited description, low production rate, and pressure.
Thus, in this contribution, we report a parahydrogen generator (PHG) based on lN2 that operates at a pressure of up to 50 bar at a cost of less than EUR 3000. The setup is easy to replicate as it is fully open source (Ellermann, 2020b) and all parts are either off-the-shelf or 3D-printed or can be constructed easily. Besides, we introduce an automated pH2 quantification method using a 1 T benchtop NMR and a microcontroller-based process control (Arduino).
In 1933 Werner Heisenberg received his Nobel Prize “for the creation of quantum mechanics, the application of which has, inter alia, led to the discovery of the allotropic forms of hydrogen” (NobelPrize.org, 2020). Allotropy is a property of substances to exist in several forms in the same physical state. Two forms of hydrogen usually are referred to as nuclear spin isomers; they are parahydrogen (pH2) and orthohydrogen (oH2). Hydrogen is not the only compound that has stable or long-lived spin isomers at room temperature (rt); there are many examples: deuterium (Knopp et al., 2003), water (Kravchuk et al., 2011; Vermette et al., 2019), ethylene (Zhivonitko et al., 2013), and methyl groups (Meier et al., 2013). Although some molecules are not symmetric and cannot be extracted at room temperature, they possess long-lived spin states of minutes (Pileio et al., 2008) and hours (Stevanato et al., 2015).
The spin of hydrogen nuclei (of protons) is the origin of the two nuclear spin-isomer forms of dihydrogen. Protons have spin-1/2; hence, they are fermions. Fermions are particles that follow the Fermi–Dirac statistics; therefore, the sign of the total wave function of H2 has to change when two nuclei are exchanged. The spin space of two spin-1/2 consists of states. They are three symmetric spin states, , , , and one asymmetric nuclear spin state, (Fig. 1). Here, conventionally α〉 and β〉 states are nuclei spin states with the projection of spin on the Z axis 1/2 and , T+〉, T0〉, T−〉 are triplet spin states of two spin-1/2 with a total spin of 1 and the projection on the Z axis +1, 0, and −1, and S〉 is a singlet spin state of two spin-1/2 with a total spin of 0.
The rotational wave function after nuclei permutation does not change, because of the molecular symmetry, and is only multiplied by (−1)J, with J being the rotational quantum number of the state. Hence, H2 with a symmetric nuclear spin state (triplet states) can only have an asymmetric rotational state (J is odd); such H2 is called oH2, and vice versa, H2 with an asymmetric nuclear spin state (singlet state) can only have symmetric rotation states (J is even); such H2 is called pH2.
The difference in the energy levels of two ground states of ortho (J=1) and para (J=0) hydrogen is (Fig. 1) (Atkins and De Paula, 2006). Such a big energy gap allows a relatively simple way of spin-isomer enrichment: for H2 the ground state is pH2 and its population can be increased by cooling down the gas (Fig. 2) (M. Richardson et al., 2018). The ratio of the number of molecules of pH2, , to oH2, , in thermal equilibrium is given by the Boltzmann distribution of rotational energy levels:
Since only two nuclear spin-isomer states of H2 exist, their fractions can be easily obtained: and . At room temperatures (T≅298 K) is close to 1:3, at 77 K – the normal boiling point of nitrogen – the ratio is close to 1:1, and at 25 K (Fig. 2).
a Depends on choice of coolant. b Estimated average flow (3.5 L volume filled to 3 bar in 90 min) calculated by us. c Estimated average flow (0.6 L volume filled to 1 bar in 240 min) calculated by us. d Highest average flow on filling 1 L bottle to 10 bar without sacrificing enrichment. e 50-bar pH2 delivery was tested. Used parts allow pressure of at least 100 bar (safety margin). f Including H2-gas sensor and excluding H2 and N2 bottles/regulators.
1.2 Technology review
pH2 fraction, , of 90 % and above is produced by PHGs with single- or dual-stage cryostats run by helium compressors. A single-stage cryostat was reported to operate at 36–40 K with a flow rate of 0.2 SLM (standard litres per minute), 10-bar maximum delivery pressure, and %–92 % (Bruker, Billerica, USA); dual-stage cryostats operate at temperatures below 25 K, where reaches 100 % (note that the boiling point of H2 is 21 K) (Haynes, 2011). All these PHGs were specifically designed with PHIP (parahydrogen-induced polarization) in mind, meaning for a relatively low scale of production and in-lab use (not for industry). These setups required some on-site assembly and were realized in different designs, e.g. with pulsed injection (Feng et al., 2012) or continuous flow (Hövener et al., 2013). The continuous flow setup was reported to operate at a conversion temperature of 25 K, a 4 SLM flow rate, a 50-bar maximum delivery pressure, and an experimentally obtained (Hövener et al., 2013).
These setups work reliably and do not require the supply of liquid cryogens. Disadvantages, however, include high initial investments (EUR 40 000–150 000), some maintenance of the He compressor and cryostat (≈ EUR 10 000 every 25 000 h operational time), some site requirements (∼4 kW cooling water, appropriate safety precautions), and operational cost in the form of electricity (>4 kW electrical power) (Table 1).
A 100 % pH2 enrichment, however, may not always be needed: 50 % pH2 fraction already provides one-third of the maximum polarization at one-tenth of the cost (or less) (M. Richardson et al., 2018). To achieve of 50 %, 77 K, the temperature of lN2, is sufficient. Indeed, lN2-based PHGs are still used in many studies (Kiryutin et al., 2017; Meier et al., 2019). The design of such PHGs is generally simple – a catalyst chamber or tube immersed in lN2, but just like cryostat-based PHGs, lN2-based PHGs are continuously improving. As such, recent advances included a remarkable work where 20 L lN2 was sufficient to provide pH2 continuously for 20 d (Jeong et al., 2018).
Interestingly, in various cases it was demonstrated that an increased flow rate and pressure of pH2 can boost the signal of PHIP or signal amplification by reversible exchange (SABRE) (Adams et al., 2009; Rayner and Duckett, 2018) beyond the factor of 3 offered by PHGs, with close to (Colell et al., 2017; Rayner et al., 2017; Štěpánek et al., 2019; Truong et al., 2015).
2.1 3D design of PHG
The principal scheme of the lN2-based PHG presented here consists of a H2 gas supply, a generator, and a pH2 storage (Fig. 3). A model of the PHG was designed (Autodesk Inventor 2019, San Rafael, USA). Aluminium profiles and steel angles (30 mm, Bosch Rexroth, Stuttgart, Germany) were used to construct the chassis. Copper tubes (outer diameter 6 mm, inner diameter 4 mm, rated for 229 bar, R220, Landefeld, Kassel-Industriepark, Germany) and valves (Swagelok, Solon, USA) were mounted on the chassis using 3D-printed parts (Ultimaker PLA “Perlweiss” Filament, Ultimaker S5, Ultimaker Cura, Utrecht, Netherlands). A 2 L stainless steel dewar was placed in the chassis (DSS 2000, 2 L, KGW Isotherm, Karlsruhe, Germany). The same copper tubes were used to wind a coil with 5.4 turns and a diameter of 86 mm. About 1.5 mL granular Fe(OH)O (371254-50G, Sigma-Aldrich, St. Louis, USA) was filled into the coil. On both ends of the copper coil, cotton wool was pressed to keep the catalyst in place to protect the rest of the system from contaminations. The compressed wool insets have a length of 20 mm. Wool as a particulate filter was used before in another PHG (Du et al., 2020). During the 6 months of weekly use of our generator, there was no sign of a moving catalyst. All fittings, T-pieces, ball valves, an overpressure valve, flow regulators, a pressure gauge, and fast connectors (Swagelok, Solon, USA) were connected with the same copper tube. For the storage of pH2, a 1 L cylinder made from aluminium was used (C1, A6341Q, Luxfer, Nottingham, UK). All parts were chosen to be rated for 100 bar or more to allow for a 100 % safety margin. A list of all parts is given in Appendix A. The models of the PHG, 3D-printing parts, and experimental macros (experimental protocols) are available (Ellermann, 2020b).
2.2 Safety concept
A crucial part of a PHG is the development of a safety concept which includes a detailed risk assessment and comprehensive operating manual. The handling of pressurized H2 gas entails the risk of pressured gases, forming a potentially explosive mixture with air as well as hydrogen embrittlement (Beeson and Woods, 2003; National Aeronautics and Space Administration, 1997). To reduce these risks, the following safety requirements were set.
Safety by design
Pressure ratings of parts
All components in contact with pressurized gas are rated for a minimum of 100 bar
Mechanical pressure gauge
100-bar safety valve for overpressure control
Avoidance of formation of explosive H2–air mixture and potential ignition
Reduction of H2 in the system by minimizing the inner volume of the gas lines
No electrical components in the system
Avoidance of temperatures above flame point
Avoidance of inductive and static spark charges in the gas lines (due to conductive and groundable pipe material)
High H2 throughput and storage of pH2 in small cylinders lead to a short operating time of PHG
Easy maintenance due to the simple and open design concept
Safety by site and operation
Strong ventilation in the installation site
No public access
Appropriate warning signs
Usage by trained personnel according to manual only
Use of safety goggles and safety gloves for the handling of lN2
H2 sensor (for leakage alarm at 50 ppm H2 level)
Regular inspection and maintenance
2.3 Production protocol
All pH2 batches were produced in the same manner (the indices in the brackets relate to Fig. 3).
Set initial state: close valve A4, connect the generator with the output via A6 (“fill” position)
Open and set supply of hydrogen to the appropriate pressure
Connect storage bottle A8 to the output
Fill the dewar with lN2 and close the lid to reduce evaporation
Wait for 20 min and set the flow with regulator A5
Flush storage bottle
Open valve A4 and wait until the pressure gauge shows 3 bar
Release gas from the storage cylinder by connecting the storage bottle to the exhaust via A6 (“venting position”)
Repeat the flushing steps three times
Production and storage of pH2
Set valve A6 to “fill” position
Wait until the gauge shows the desired pressure
Close valve A4
Finishing production of pH2
Close storage bottle (bottle valve)
Close H2 supply
Set valve A6 to “vent” position to reduce pressure in the output line
Disconnect storage bottle from the output (fast connect adapters keep the line closed) and connect an evacuated bottle or a bottle with a low H2 pressure
Set valve A6 to “close” position
Ensure that the PHG is left with 2–5 bar of H2
2.4.1 Flow quantification
We refrained from including a flow meter in the setup to keep it simple and robust. Instead, we used the time tp,V needed to fill a cylinder of a given volume V0 to a given pressure ptarget to measure the average flow rate fr of the pH2 production. The pressure pout in the outlet of the PHG increases during production. To obtain SLM, we used the following equation:
where Trt is the temperature of the quantification experiment (here: 22 ∘C) and “stand” stands for standard pressure and temperature values (pstand=105 Pa, Tstand=273.15 K) (Nič et al., 2009). The measurement of fr is performed in a regime where pout is linear as a function of time (tp,V), and hence it coincides with the initial flow rate that is usually reported.
2.4.2 Gas system
A medium-pressure 5 mm NMR tube (522-QPV-8, Wilmad-LabGlass, Vineland, USA) was used for the pH2 quantification and a heavy wall 5 mm NMR tube (Wilmad-LabGlass, 522-PV-9) for experiments with magnetic field cycling (MFC). Each of these NMR tubes was equipped with input and output gas lines (1/16′′ polytetrafluoroethylene capillary (PTFE) with 0.023′′ inner diameter) by gluing them to the cap. The other end of these tubes was connected to a custom-made valve system. The pressure in the system was set by changing the reducers of the respective gases and the back-pressure valve in the gas system (P-785, P-787, Postnova). The inlet gas pressure was regulated to achieve a steady bubbling for the given back pressures of 2.8 bar and 6.9 bar. The valve system is controlled with a microcontroller (Arduino) which was linked to the spectrometer software synchronizing the gas supply, venting of the NMR tube, and data acquisition. Using this gas system, we supplied the NMR tube with N2 (99.999 %, Air Liquide), H2 (99.999 %, Air Liquide), or pH2.
2.4.3 pH2 quantification protocol
The pH2 quantification was performed according to a quantification protocol (schematically shown in Fig. 4).
A NMR tube was placed in a 1 T NMR spectrometer (benchtop, SpinSolve Carbon 43 MHz, Magritek, Aachen, Germany) and not moved during the experiment. To remove air and residual gases from the lines, the setup was flushed with the gas for 3 min at a 5-bar input pressure and a fully open exhaust. Afterwards, the exhaust line was closed and a 30 s delay was allowed to stabilize pressure and flow before the NMR acquisition was started. To ensure constant pressure in the system, the gas supply was kept open during the NMR measurement. Because the NMR signal was not locked during the experiment, the H2 resonance was moved to 0 ppm during post-processing for convenience.
All NMR spectra of gases were acquired with a standard excitation and acquisition pulse sequence (12.6 µs excitation pulse that corresponds to 90∘ flip angle, 20 ms acquisition time, 50 kHz spectral width, 0.5 s repetition time, 100 transients for averaging, SpinSolve Expert v3.54, Magritek, Aachen, Germany). The spectra were subjected to 20 Hz exponential apodization and phase correction. To remove background signals, a spectrum of N2 was also acquired and subtracted from the rtH2 (H2 in thermal equilibrium at room temperature) and pH2 spectra. After that, an automatic baseline correction (MNova v14.1.2, Santiago de Compostela, Spain) was applied to the phased spectrum. The spectral lines of rtH2 and pH2 were integrated within the borders of −15 and +15 ppm, giving S(rtH2) and S(pH2). Finally the fraction of pH2 was calculated:
Here we take into account that only oH2 contributes to the MR signal and at room temperature (Green et al., 2012).
2.5 SABRE experiment
2.5.1 Sample preparation
The sample solution contained 3 mmol/L iridium N-heterocyclic carbene complex [Ir(COD)(IMes)Cl], where COD = 1,5-cyclooctadiene and Imes = 1,3-bis(2,4,6-trimethylphenyl) imidazol-2-ylidene (Cowley et al., 2011) and 26 mmol/L nicotinamide (CAS 98-92-0, Sigma-Aldrich) in methanol-d4 99.8 % (Deutero GmbH). To activate the catalyst, H2 was flushed through the sample at 6.9 bar for 5 min before the magnetic field cycling experiments began.
2.5.2 Magnetic field cycling experiment
The NMR spectrometer was equipped with an in-house built MFC setup that will be described elsewhere. The shuttling time from the observation point to the homogenous area of the electromagnet was 0.2 s. The used electromagnet allowed a magnetic field variation in the range of −20 to +20 mT with a magnetic field homogeneity of 0.06 % in 2 cm. The same gas system as described above was used for the MFC SABRE experiments. The only modification was that a hollow optical fibre (Molex, part. no. 106815-0026, 250 µm internal diameter, 360 µm outer diameter) was glued to the end of the PTFE capillaries to reduce magnetic field distortions. All magnetic field cycling SABRE experiments were carried out according to the protocol in Fig. 5.
3.1 PHG design
A PHG fulfilling the initial design requirements was successfully constructed (Fig. 6). Most parts were either commercially available, 3D-printed or simple to construct on-site. The holders for the bottles and a bottom plate were the sole parts prepared by a mechanical workshop. All parts were rated for more than 100 bar and no H2 leaks were detected at 50 bar of H2 using a H2 detector (GasBadge Pro H2, Industrial Scientific, Pittsburgh, USA). Inspection and operation were facilitated by easy access and open construction design. The total cost was below EUR 3000 (Appendix A).
We deliberately abstained from including a flow meter in the setup to keep the cost low and increase the robustness. Instead, we monitored the pressure pout in the storage cylinder and calculated the flow rate (Fig. 7a). The expected increase in pressure and decrease in the flow rate of pH2 were observed. The flow rate is an important parameter since it affects the number of H2 collisions with the catalyst in the ortho-para-conversion unit (Figs. 3, A3) that enables fast para-ortho conversion. A lN2-based PHG can provide % at maximum (Fig. 2, 7b). If the flow rate is too fast, the gas will not have enough time to reach the ortho-para thermal equilibrium while passing through the unit. Hence the pH2 fraction will be reduced.
Thus, to find optimal performance conditions of the PHG, we quantified as a function of the flow rate (Fig. 7c) set by the needle valves (Fig. 3, A5). At the given settings of pin=20 bar and ptarget=10 bar, % was found for a flow up to fr=2 SLM. For larger flow rates, the enrichment dropped significantly. Given these data and to allow for some variation, we chose a standard operating flow of ∼0.9 SLM. This flow rate was fast enough for convenient pH2 production. For example, l L of 49 bar pH2 with ) % was produced in 29 min (pin=49 bar, initial flow rate of 2.9 SLM, Fig. 7a).
3.2 The precision of pH2 production, quantification and lifetime
To test the reproducibility of the quantification method, of a single batch was quantified five times in a row (including venting, flushing, and filling of the tube). The average was found to be (51.5±0.4) %, corresponding to a coefficient of variation (CV) of 0.7 % (Fig. 8).
To access the reproducibility of the entire production process, four pH2 batches were produced on different days and quantified. An average of (51.6±0.9) % was observed (CV = 1.7 %) (Fig. 8).
We also investigated as a function of the inlet pressure pin at a fixed flow rate: a batch was prepared for pin equal to 12, 20, 35, and 50 bar, ptarget=10 bar, and a flow rate of 0.9 SLM. No pressure dependency was observed. The obtained average of was (52.4±0.8) %.
To evaluate the lifetime of pH2 in the 2 L cylinder, a 10-bar pH2 batch was produced (pin=20 bar, fr=0.9 SLM). Over 22 d, five samples were taken from the batch and was quantified. An exponential decay function was fitted to the data and yielded a constant of 35.5±1.5 d (Fig. 9).
3.3 Application: 1H-low-field SABRE at different pH2 pressures
The presented setup was designed to allow for pressures up to 50 bar. High pressures are beneficial for hyperpolarization because the concentration of pH2 in the solution increases with pressure. A low concentration of pH2 is often the limiting factor of the hyperpolarization payload (polarization level × concentration of polarized species). To demonstrate the effect, we polarized nicotine amide by SABRE and magnetic field cycling (scheme in Fig. 5) at two different pH2 pressures: 2.8 and 6.9 bar (Fig. 10). Strong polarization was observed on the 1H resonances of nicotine amide and hydrogen in solution. A 2.5-fold increase in pressure yielded a 2.3-fold increase in nicotine amide polarization.
The design of the presented PHG is simple and compact without compromising on performance and safety. The PHG is small and portable (although a heavy bottom plate was added for stability). Since there are no electrical components, it can be placed indoors as well as outdoors and does not require any electrical power supply. Note that electric components can be an ignition source which may lead to an explosion in case of a hydrogen leak.
For the framework, mostly off-the-shelf parts were used. More complex geometries, e.g. holders for valves or gauges, were 3D-printed. They have individual shapes and dimensions, and manufacturing in a workshop might lead to high costs and long production lead times. Three-dimensional printing turned out to be a versatile manufacturing method enabling fast prototyping, complex shapes, and low cost for one-off productions. The design of the PHG and all 3D models (STL files, Standard Triangulation Language, and CAD files, computer-aided design) are provided, enabling other groups to adjust the parts to their individual needs (Ellermann, 2020b).
Choosing a small 2 L dewar keeps the design compact and the running costs low since less than 2 L of liquid nitrogen was required to prepare 1 L of pH2 at 50 bar. In combination with a short cooling down time, the setup is well suited for an on-site pH2 production in a hyperpolarization lab.
The final cost of the PHG incl. the hydrogen sensor was EUR 2988 incl. VAT (19 %). If a hydrogen sensor is already available in the lab, the overall costs for the PHG may be less than EUR 2500 incl. VAT (19 %). A complete set including the PHG, a hydrogen sensor, hydrogen/nitrogen gas as well as a variety of essential tools cost about EUR 3700 incl. VAT (19 %).
All parts which are in contact with pressurized gas are rated to at least 100 bar. However, we fixed the operation pressure to 50 bar to get a generous safety margin of 100 %. The risk of static and inductive spark charges in the gas line is low (Department of Labour of New Zealand, 1990). Nevertheless, the gas pipes can be grounded to prevent electrical charges on the parts which are in contact with H2 gas.
The design of the PHG incorporates a gas path which also enabled safe ventilation of a storage bottle. Potential handling errors were also considered. For example, the output connectors are closed for pressures up to 17 bar when they are disconnected, i.e. the case when the storage bottle is disconnected. Thus, there is no contact between the air in the room and the hydrogen in the PHG. Furthermore, we included a handheld hydrogen sensor that measures a hydrogen concentration as low as parts per million. The sensor should be always turned on during operation to indicate a potential leak of H2.
The setup includes low-temperature cryogens such as liquid nitrogen. To prevent the spilling of liquid nitrogen, the dewar was restrained by the copper tubing inside and the surrounding metal frame. Moreover, a lid covered the liquid nitrogen bath that also reduced the evaporation rate of the cryogen. Since the flask only holds around 2 L of cryogen, the amount of liquid nitrogen that has to be handled is greatly reduced.
Note that the PHG should be placed in a non-public restricted-access enclosure or room with sufficient ventilation and that only instructed personnel should operate it.
Our presented safety concept is economical and practical without sacrificing any safety measures. Nevertheless, after setting up the PHG, it should be tested for leakage with an inert gas or nitrogen. The part list also contains a leak detection spray and the H2 sensor.
The enrichment achieved here, e.g. % for pin=20 bar, fr=0.9 SLM, was close to the maximum of 51.8 % conditioned by the boiling point of lN2 and somewhat higher than reported elsewhere: = 50 % (Barskiy et al., 2016a, 2016b; Shchepin et al., 2016). Determining the enrichment as a function of flow allowed us to choose an optimal flow of 0.9 SLM for pin=20 bar: this rate was e.g. sufficient to fill 1 L bottles to 10 bar in 10 min. The central design criterion of high pressure was successfully met as 1 L of 49 bar pH2 was produced in 28 min (pin=50 bar). We demonstrated that an increase in pH2 pressure can give a proportional increase in polarization (Fig. 10). Obviously, this approach is limited as soon as the hyperpolarization yield is no longer determined by the availability of pH2 and cannot provide a polarization above 33 % (Korchak et al., 2018).
4.1.4 pH2 quantification and production reliability
The automatic quantification process featured a CV of 0.7 %; the pH2 production and quantification together featured a CV of 1.7 % (Fig. 8). These results indicate that the routine pH2 quality control can be performed with a low-cost 1 T benchtop NMR spectrometer. The automatization certainly helped to make the process more reliable but was not necessary. Feng et al. (2012) used the similar quantification approach and reported a precision of 1 %–3 % for quantification. NMR is a convenient method for pH2 quantification, but optical methods may be used, too (Parrott et al., 2019).
4.1.5 The lifetime in aluminium cylinder
The relaxation time constant in aluminium tanks was found to be (63.7±8.3) d by Feng et al. (2012) and ∼120 d by Hövener et al. (2013), respectively. We found here a shorter lifetime of (35.5±1.5) d in our 2 L aluminium storage bottle. Note that we did not perform any dedicated cleaning procedure for the pH2 storage bottle. Still, the lifetime was sufficiently long to produce pH2 once a week.
The presented PHG enables the production of pH2 with % at a high pressure of 50 bar reliably (CV = 1.7 %), providing about one-third of the polarization achieved with ≈ 100 %. Because the device delivers high-pressure pH2, however, this effect can be partially compensated in the PHIP/SABRE experiment. A new, automated quantification routine at 1 T benchtop NMR proved to be reliable and simple (CV = 0.7 %). The design of the PHG is straightforward and easy to manufacture with openly available blueprints and at a cost of less than EUR 3000. The device may facilitate further research on the promising method of parahydrogen-based hyperpolarization.
∗ Overall costs may vary due to change in prices and change in VAT rate and due to costs which may arise for custom parts (e.g. material or labour costs from the facility's workshop).
Software for automatic signal recording and gas control will be available from figshare.com (DOI: https://doi.org/10.6084/m9.figshare.13176830, Ellermann et al., 2020a) and via git (https://gitlab.tardis.rad.uni-kiel.de/fellermann/opensource-liquid-n2-based-ph2-generator) (Ellermann et al., 2020b).
All experimental data (SpinSolve 1H NMR spectra of H2) and blueprints for the PHG will be available from figshare.com (DOI: https://doi.org/10.6084/m9.figshare.13176830) (Ellermann et al., 2020a). Additionally, all blueprints are also accessible via git (https://gitlab.tardis.rad.uni-kiel.de/fellermann/opensource-liquid-n2-based-ph2-generator) (Ellermann et al., 2020b).
Data curation, investigation, formal analysis, software development (here programming of macros), validation, visualization, and writing of the original draft were done by FE. AP and JBH contributed equally to conceptualization, supervision, and reviewing the manuscript.
The authors declare that they have no conflict of interest.
We are grateful for support by the Emmy Noether Program “metabolic and molecular MR” (grant no. HO 4604/2-2), the research training circle “materials for brain” (grant no. GRK 2154/1-2019), the DFG (grant no. HO 4604/3-1), the RFBR (grant no. 19-53-12013), the German Federal Ministry of Education and Research (BMBF) within the framework of the e:Med research and funding concept (grant no. 01ZX1915C), and Cluster of Excellence “precision medicine in inflammation” (grant no. PMI 1267). Kiel University and the Medical Faculty are thanked for supporting the Molecular Imaging North Competence Center (MOIN CC) as a core facility for imaging in vivo. MOIN CC was founded by a grant from the European Regional Development Fund (ERDF) and the Zukunftsprogramm Wirtschaft of Schleswig-Holstein (grant no. 122-09-053).
This research has been supported by the German Research Foundation (DFG) (grant nos. GRK 2154/1-2019, HO 4604/2-2, and HO 4604/3-1), the German Federal Ministry of Education and Research (BMBF) (grant no. 01ZX1915C), the RFBR (grant no. 19-53-12013), Cluster of Excellence “precision medicine in inflammation” (no. PMI 1267), and the Zukunftsprogramm Wirtschaft of Schleswig-Holstein (grant no. 122-09-053). The Molecular Imaging North Competence Center (MOIN CC) was funded by a grant from the European Regional Development Fund (ERDF) and is supported by Kiel University and the medical faculty of Kiel University.
This paper was edited by Sami Jannin and reviewed by James Eills, Eleonora Cavallari, and one anonymous referee.
Adams, R. W., Aguilar, J. A., Atkinson, K. D., Cowley, M. J., Elliott, P. I. P., Duckett, S. B., Green, G. G. R., Khazal, I. G., López-Serrano, J., and Williamson, D. C.: Reversible Interactions with para-Hydrogen Enhance NMR Sensitivity by Polarization Transfer, Science, 323, 1708–1711, https://doi.org/10.1126/science.1168877, 2009.
Atkins, P. W. and De Paula, J.: Physical chemistry, Oxford University Press, Oxford, New York, 2006.
Barskiy, D. A., Salnikov, O. G., Shchepin, R. V., Feldman, M. A., Coffey, A. M., Kovtunov, K. V., Koptyug, I. V., and Chekmenev, E. Y.: NMR SLIC Sensing of Hydrogenation Reactions Using Parahydrogen in Low Magnetic Fields, J. Phys. Chem. C, 120, 29098–29106, https://doi.org/10.1021/acs.jpcc.6b07555, 2016a.
Barskiy, D. A., Shchepin, R. V., Coffey, A. M., Theis, T., Warren, W. S., Goodson, B. M., and Chekmenev, E. Y.: Over 20% 15N Hyperpolarization in Under One Minute for Metronidazole, an Antibiotic and Hypoxia Probe, J. Am. Chem. Soc., 138, 8080–8083, https://doi.org/10.1021/jacs.6b04784, 2016b.
Beek, E. J. R. van, Wild, J. M., Kauczor, H.-U., Schreiber, W., Mugler, J. P., and de Lange, E. E.: Functional MRI of the lung using hyperpolarized 3-helium gas, J. Mag. Reson. Imag., 20, 540–554, https://doi.org/10.1002/jmri.20154, 2004.
Beeson, H. and Woods, S.: Guide for Hydrogen Hazards Analysis on Components and Systems, available at: https://ntrs.nasa.gov/api/citations/20040033949/downloads/20040033949.pdf (last access: 14 January 2021), Comp. Syst., 40, 1–3, 2003.
Birchall, J. R., Coffey, A. M., Goodson, B. M., and Chekmenev, E. Y.: High-Pressure Clinical-Scale 87% Parahydrogen Generator, Anal. Chem., 92, 15255–15684, https://doi.org/10.1021/acs.analchem.0c03358, 2020.
Bowers, C. R. and Weitekamp, D. P.: Transformation of Symmetrization Order to Nuclear-Spin Magnetization by Chemical Reaction and Nuclear Magnetic Resonance, Phys. Rev. Lett., 57, 2645–2648, https://doi.org/10.1103/PhysRevLett.57.2645, 1986.
Bowers, C. R. and Weitekamp, D. P.: Parahydrogen and synthesis allow dramatically enhanced nuclear alignment, J. Am. Chem. Soc., 109, 5541–5542, https://doi.org/10.1021/ja00252a049, 1987.
Buckenmaier, K., Rudolph, M., Fehling, P., Steffen, T., Back, C., Bernard, R., Pohmann, R., Bernarding, J., Kleiner, R., Koelle, D., Plaumann, M., and Scheffler, K.: Mutual benefit achieved by combining ultralow-field magnetic resonance and hyperpolarizing techniques, Rev. Sci. Instrum., 89, 125103, https://doi.org/10.1063/1.5043369, 2018.
Colell, J. F. P., Logan, A. W. J., Zhou, Z., Shchepin, R. V., Barskiy, D. A., Ortiz, G. X., Wang, Q., Malcolmson, S. J., Chekmenev, E. Y., Warren, W. S., and Theis, T.: Generalizing, Extending, and Maximizing Nitrogen-15 Hyperpolarization Induced by Parahydrogen in Reversible Exchange, J. Phys. Chem. C, 121, 6626–6634, https://doi.org/10.1021/acs.jpcc.6b12097, 2017.
Cowley, M. J., Adams, R. W., Atkinson, K. D., Cockett, M. C. R., Duckett, S. B., Green, G. G. R., Lohman, J. A. B., Kerssebaum, R., Kilgour, D., and Mewis, R. E.: Iridium N-Heterocyclic Carbene Complexes as Efficient Catalysts for Magnetization Transfer from para-Hydrogen, J. Am. Chem. Soc., 133, 6134–6137, https://doi.org/10.1021/ja200299u, 2011.
Department of Labour of New Zealand: Guidelines for the CONTROL OF STATIC ELECTRICITY INDUSTRY, available at: http://www-eng.lbl.gov/~shuman/NEXT/GAS_SYS/staticelectricity.pdf (last access: 24 November 2020), 1990.
Du, Y., Zhou, R., Ferrer, M.-J., Chen, M., Graham, J., Malphurs, B., Labbe, G., Huang, W., and Bowers, C. R.: An Inexpensive Apparatus for up to 97% Continuous-Flow Parahydrogen Enrichment Using Liquid Helium, J. Mag. Reson., 321, 106869, https://doi.org/10.1016/j.jmr.2020.106869, 2020.
Eisenschmid, T. C., Kirss, R. U., Deutsch, P. P., Hommeltoft, S. I., Eisenberg, R., Bargon, J., Lawler, R. G., and Balch, A. L.: Para hydrogen induced polarization in hydrogenation reactions, J. Am. Chem. Soc., 109, 8089–8091, https://doi.org/10.1021/ja00260a026, 1987.
Ellermann, F., Pravdivtsev, A. N., and Hövener, J.-B.: Para-hydrogen generator files, Figshare, https://doi.org/10.6084/m9.figshare.13176830.v1, 2020a.
Ellermann, F., Pravdivtsev, A. N., and Hövener, J.-B.: git: opensource-liquid-N2-based pH2 Generator, available at: https://gitlab.tardis.rad.uni-kiel.de/fellermann/opensource-liquid-n2-based-ph2-generator (last access: 31 October 2020), 2020b.
Feng, B., Coffey, A. M., Colon, R. D., Chekmenev, E. Y., and Waddell, K. W.: A pulsed injection parahydrogen generator and techniques for quantifying enrichment, J. Magn. Reson., 214, 258–262, https://doi.org/10.1016/j.jmr.2011.11.015, 2012.
Feyter, H. M. D., Behar, K. L., Corbin, Z. A., Fulbright, R. K., Brown, P. B., McIntyre, S., Nixon, T. W., Rothman, D. L., and Graaf, R. A. de: Deuterium metabolic imaging (DMI) for MRI-based 3D mapping of metabolism in vivo, Sci. Adv., 4, eaat7314, https://doi.org/10.1126/sciadv.aat7314, 2018.
Gamliel, A., Allouche-Arnon, H., Nalbandian, R., Barzilay, C. M., Gomori, J. M., and Katz-Brull, R.: An Apparatus for Production of Isotopically and Spin-Enriched Hydrogen for Induced Polarization Studies, Appl. Magn. Reson., 39, 329–345, https://doi.org/10.1007/s00723-010-0161-9, 2010.
Green, R. A., Adams, R. W., Duckett, S. B., Mewis, R. E., Williamson, D. C., and Green, G. G. R.: The theory and practice of hyperpolarization in magnetic resonance using parahydrogen, Prog. Nucl. Magn. Reson. Spectrosc., 67(Supplement C), 1–48, https://doi.org/10.1016/j.pnmrs.2012.03.001, 2012.
Haynes, W. M.: CRC Handbook of Chemistry and Physics, in CRC Handbook of Chemistry and Physics, 4.121–4.123, CRC Press, New York, NY, 2011.
Hövener, J.-B., Bär, S., Leupold, J., Jenne, K., Leibfritz, D., Hennig, J., Duckett, S. B., and von Elverfeldt, D.: A continuous-flow, high-throughput, high-pressure parahydrogen converter for hyperpolarization in a clinical setting, NMR Biomed., 26, 124–131, https://doi.org/10.1002/nbm.2827, 2013.
Hövener, J.-B., Pravdivtsev, A. N., Kidd, B., Bowers, C. R., Glöggler, S., Kovtunov, K. V., Plaumann, M., Katz-Brull, R., Buckenmaier, K., Jerschow, A., Reineri, F., Theis, T., Shchepin, R. V., Wagner, S., Bhattacharya, P., Zacharias, N. M., and Chekmenev, E. Y.: Parahydrogen-Based Hyperpolarization for Biomedicine, Angew. Chem. Int. Edit., 57, 11140–11162, https://doi.org/10.1002/anie.201711842, 2018.
Jeong, K., Min, S., Chae, H., and Namgoong, S. K.: Detecting low concentrations of unsaturated C-C bonds by parahydrogen-induced polarization using an efficient home-built parahydrogen generator, Magn. Reson. Chem., 56, 1089–1093, https://doi.org/10.1002/mrc.4756, 2018.
Kiryutin, A. S., Sauer, G., Hadjiali, S., Yurkovskaya, A. V., Breitzke, H., and Buntkowsky, G.: A highly versatile automatized setup for quantitative measurements of PHIP enhancements, J. Magn. Reson., 285(Supplement C), 26–36, https://doi.org/10.1016/j.jmr.2017.10.007, 2017.
Knopp, G., Kirch, K., Beaud, P., Mishima, K., Spitzer, H., Radi, P., Tulej, M., and Gerber, T.: Determination of the ortho-/para deuterium concentration ratio with femtosecond CARS, J. Raman Spectrosc., 34, 989–993, https://doi.org/10.1002/jrs.1091, 2003.
Korchak, S., Mamone, S., and Glöggler, S.: Over 50 % 1H and 13C Polarization for Generating Hyperpolarized Metabolites – A para-Hydrogen Approach, ChemistryOpen, 7, 672–676, https://doi.org/10.1002/open.201800086, 2018.
Kovtunov, K. V., Pokochueva, E. V., Salnikov, O. G., Cousin, S. F., Kurzbach, D., Vuichoud, B., Jannin, S., Chekmenev, E. Y., Goodson, B. M., Barskiy, D. A., and Koptyug, I. V.: Hyperpolarized NMR Spectroscopy: d-DNP, PHIP, and SABRE Techniques, Chem. An Asian J., 13, 1857–1871, https://doi.org/10.1002/asia.201800551, 2018.
Kravchuk, T., Reznikov, M., Tichonov, P., Avidor, N., Meir, Y., Bekkerman, A., and Alexandrowicz, G.: A Magnetically Focused Molecular Beam of Ortho-Water, Science, 331, 319–321, https://doi.org/10.1126/science.1200433, 2011.
Kurhanewicz, J., Vigneron, D. B., Brindle, K., Chekmenev, E. Y., Comment, A., Cunningham, C. H., DeBerardinis, R. J., Green, G. G., Leach, M. O., Rajan, S. S., Rizi, R. R., Ross, B. D., Warren, W. S., and Malloy, C. R.: Analysis of Cancer Metabolism by Imaging Hyperpolarized Nuclei: Prospects for Translation to Clinical Research, Neoplasia, 13, 81–97, https://doi.org/10.1593/neo.101102, 2011.
Lange, O. F., Lakomek, N.-A., Farès, C., Schröder, G. F., Walter, K. F. A., Becker, S., Meiler, J., Grubmüller, H., Griesinger, C., and de Groot, B. L.: Recognition Dynamics Up to Microseconds Revealed from an RDC-Derived Ubiquitin Ensemble in Solution, Science, 320, 1471–1475, https://doi.org/10.1126/science.1157092, 2008.
Meier, B., Dumez, J.-N., Stevanato, G., Hill-Cousins, J. T., Roy, S. S., Håkansson, P., Mamone, S., Brown, R. C. D., Pileio, G., and Levitt, M. H.: Long-Lived Nuclear Spin States in Methyl Groups and Quantum-Rotor-Induced Polarization, J. Am. Chem. Soc., 135, 18746–18749, https://doi.org/10.1021/ja410432f, 2013.
Meier, B., Kouril, K., and Kourilova, H.: Para-Hydrogen Generator. Low-cost para-hydrogen for anyone, available at: http://www.hyperspin.biz/#phip (last access: 16 October 2020), 2019.
National Aeronautics and Space Administration: Safety Standard for Hydrogen and Hydrogen Systems, available at: https://www.energy.gov/sites/prod/files/2014/03/f11/871916.pdf (last access: 12 August 2020), NSS 1740.16, 1997.
Nič, M., Jirát, J., Košata, B., Jenkins, A., and McNaught, A., Eds.: standard conditions for gases, in: IUPAC Compendium of Chemical Terminology, IUPAC, Research Triagle Park, NC, 2009.
NobelPrize.org: The Nobel Prize in Physics 1932, NobelPrize.org, available at: https://www.nobelprize.org/prizes/physics/1932/summary/, last access: 16 October 2020.
Parrott, A. J., Dallin, P., Andrews, J., Richardson, P. M., Semenova, O., Halse, M. E., Duckett, S. B., and Nordon, A.: Quantitative In Situ Monitoring of Parahydrogen Fraction Using Raman Spectroscopy, Appl. Spectrosc., 73, 88–97, https://doi.org/10.1177/0003702818798644, 2019.
Pileio, G., Carravetta, M., Hughes, E., and Levitt, M. H.: The long-lived nuclear singlet state of 15N-nitrous oxide in solution, J. Am. Chem. Soc., 130, 12582–12583, https://doi.org/10.1021/ja803601d, 2008.
Rayner, P. J. and Duckett, S. B.: Signal Amplification by Reversible Exchange (SABRE): From Discovery to Diagnosis, Angew. Chem. Intern. Edit., 57, 6742–6753, https://doi.org/10.1002/anie.201710406, 2018.
Rayner, P. J., Burns, M. J., Olaru, A. M., Norcott, P., Fekete, M., Green, G. G. R., Highton, L. A. R., Mewis, R. E., and Duckett, S. B.: Delivering strong 1H nuclear hyperpolarization levels and long magnetic lifetimes through signal amplification by reversible exchange, P. Natl. Acad. Sci., 114, E3188–E3194, https://doi.org/10.1073/pnas.1620457114, 2017.
Richardson, P. M., John, R. O., Parrott, A. J., Rayner, P. J., Iali, W., Nordon, A., Halse, M. E., and Duckett, S. B.: Quantification of hyperpolarisation efficiency in SABRE and SABRE-Relay enhanced NMR spectroscopy, Phys. Chem. Chem. Phys., 20, 26362–26371, https://doi.org/10.1039/C8CP05473H, 2018.
Shchepin, R. V., Barskiy, D. A., Coffey, A. M., Theis, T., Shi, F., Warren, W. S., Goodson, B. M., and Chekmenev, E. Y.: 15N Hyperpolarization of Imidazole-15N2 for Magnetic Resonance pH Sensing via SABRE-SHEATH, ACS Sens., 1, 640–644, https://doi.org/10.1021/acssensors.6b00231, 2016.
Silvera, I. F.: The solid molecular hydrogens in the condensed phase: Fundamentals and static properties, Rev. Mod. Phys., 52, 393–452, https://doi.org/10.1103/RevModPhys.52.393, 1980.
Štěpánek, P., Sanchez-Perez, C., Telkki, V.-V., Zhivonitko, V. V., and Kantola, A. M.: High-throughput continuous-flow system for SABRE hyperpolarization, J. Magne. Reson., 300, 8–17, https://doi.org/10.1016/j.jmr.2019.01.003, 2019.
Stevanato, G., Hill-Cousins, J. T., Håkansson, P., Roy, S. S., Brown, L. J., Brown, R. C. D., Pileio, G., and Levitt, M. H.: A Nuclear Singlet Lifetime of More than One Hour in Room-Temperature Solution, Angew. Chem. Int. Edit., 54, 3740–3743, https://doi.org/10.1002/anie.201411978, 2015.
Truong, M. L., Theis, T., Coffey, A. M., Shchepin, R. V., Waddell, K. W., Shi, F., Goodson, B. M., Warren, W. S., and Chekmenev, E. Y.: 15N Hyperpolarization by Reversible Exchange Using SABRE-SHEATH, J. Phys. Chem. C, 119, 8786–8797, https://doi.org/10.1021/acs.jpcc.5b01799, 2015.
Vermette, J., Braud, I., Turgeon, P.-A., Alexandrowicz, G., and Ayotte, P.: Quantum State-Resolved Characterization of a Magnetically Focused Beam of ortho-H2O, J. Phys. Chem. A, 123, 9234–9239, https://doi.org/10.1021/acs.jpca.9b04294, 2019.
Watson, W. D., Miller, J. J. J., Lewis, A., Neubauer, S., Tyler, D., Rider, O. J., and Valkovič, L.: Use of cardiac magnetic resonance to detect changes in metabolism in heart failure, Cardio. Diag. Ther., 10, 583–597, https://doi.org/10.21037/cdt.2019.12.13, 2020.
Wilferth, T., Gast, L. V., Lachner, S., Behl, N. G. R., Schmidt, M., Dörfler, A., Uder, M., and Nagel, A. M.: X-Nuclei MRI on a 7T MAGNETOM Terra: Initial Experiences, MAGNETOM Flash, 76, 45–51, 2020.
Xu, V., Chan, H., Lin, A., Sailasuta, N., Valencerina, S., Tran, T., Hovener, J., and Ross, B.: MR Spectroscopy in Diagnosis and Neurological Decision-Making, Semin. Neurol., 28, 407–422, https://doi.org/10.1055/s-0028-1083685, 2008.
Zhivonitko, V. V., Kovtunov, K. V., Chapovsky, P. L., and Koptyug, I. V.: Nuclear Spin Isomers of Ethylene: Enrichment by Chemical Synthesis and Application for NMR Signal Enhancement, Angew. Chem. Int. Ed., 52, 13251–13255, https://doi.org/10.1002/anie.201307389, 2013.