Analysis of conformational exchange processes using methyl-TROSY-based Hahn echo measurements of quadruple-quantum relaxation

Waudby, Christopher A.; Christodoulou, John

doi:https://doi.org/10.5194/mr-2-777-2021

Articles | Volume 2, issue 2

https://doi.org/10.5194/mr-2-777-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

https://doi.org/10.5194/mr-2-777-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 2, issue 2

Research article

|

03 Nov 2021

Research article |

| 03 Nov 2021

Analysis of conformational exchange processes using methyl-TROSY-based Hahn echo measurements of quadruple-quantum relaxation

Christopher A. Waudby and John Christodoulou

Supplement

https://doi.org/10.5194/mr-2-777-2021-supplement

Data sets

Data for: Analysis of Conformational Exchange Processes using Methyl-TROSY-Based Hahn Echo Measurements of Quadruple-Quantum Relaxation Christopher A. Waudby and John Christodoulou https://doi.org/10.5281/zenodo.5559835

Model code and software

QQ Hahn Echo and CPMG analysis Christopher A. Waudby and John Christodoulou https://doi.org/10.5281/zenodo.5559636

NMRTools.jl (v0.0.2) Christopher A. Waudby https://doi.org/10.5281/zenodo.5585089

Short summary

We describe a suite of experiments that exploit field-dependent relaxation measurements of four-spin transitions in methyl groups to characterise chemical exchange processes and which can be used as an alternative or complement to CPMG relaxation dispersion measurements. We show that these four-spin transitions benefit from the methyl TROSY effect and so provide a unique combination of slow intrinsic relaxation and high sensitivity to chemical exchange.