Articles | Volume 2, issue 2
Research article
09 Nov 2021
Research article |  | 09 Nov 2021

Fluorine NMR study of proline-rich sequences using fluoroprolines

Davy Sinnaeve, Abir Ben Bouzayene, Emile Ottoy, Gert-Jan Hofman, Eva Erdmann, Bruno Linclau, Ilya Kuprov, José C. Martins, Vladimir Torbeev, and Bruno Kieffer

Related subject area

Field: Liquid-state NMR | Topic: Applications – biological macromolecules
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Cited articles

Ahuja, P., Cantrelle, F. X., Huvent, I., Hanoulle, X., Lopez, J., Smet, C., Wieruszeski, J. M., Landrieu, I., and Lippens, G.: Proline Conformation in a Functional Tau Fragment, J. Mol. Biol., 428, 79–91,, 2016. 
Aufiero, M. and Gilmour, R.: Informing Molecular Design by Stereoelectronic Theory: The Fluorine Gauche Effect in Catalysis, Acc. Chem. Res., 51, 1701–1710,, 2018. 
Behrendt, R. P., White, P., and Offer, J.: Advances in Fmoc solid-phase peptide synthesis, J. Pept. Sci., 22, 4–27,, 2016. 
Berger, A. A., Völler, J.-S., Budisa, N., and Koksch, B.: Deciphering the Fluorine Code – The Many Hats Fluorine Wears in a Protein Environment, Acc. Chem. Res., 50, 2093–2103,, 2017. 
Best, R. B., Merchant, K. A., Gopich, I. V., Schuler, B., Bax, A., and Eaton W. A.: Effect of flexibility and cis residues in single-molecule FRET studies of polyproline, P. Natl. Acad. Sci. USA, 104, 18964–18969,, 2007. 
Short summary
Fluorine NMR was used to study the interaction between a proline-rich peptide and a SH3 domain using 4S- and 4R-fluorinated prolines whose potential as NMR probes has not been exploited yet. We present a comprehensive study addressing several aspects to be considered when using these residues as NMR probes, including relaxation and dynamics. We show that their conformational bias may be used to modulate the kinetics of protein binding to proline-rich motifs.