Articles | Volume 2, issue 2
https://doi.org/10.5194/mr-2-815-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Special issue:
https://doi.org/10.5194/mr-2-815-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
11 Nov 2021
Research article |
| 11 Nov 2021
| 11 Nov 2021
A cryogen-free, semi-automated apparatus for bullet-dynamic nuclear polarization with improved resolution
Karel Kouřil
CORRESPONDING AUTHOR
Institute for Biological Interfaces 4, Karlsruhe Institute of Technology, Karlsruhe, Germany
Michel Gramberg
Institute for Biological Interfaces 4, Karlsruhe Institute of Technology, Karlsruhe, Germany
Michael Jurkutat
Institute for Biological Interfaces 4, Karlsruhe Institute of Technology, Karlsruhe, Germany
Hana Kouřilová
Institute for Biological Interfaces 4, Karlsruhe Institute of Technology, Karlsruhe, Germany
Institute for Biological Interfaces 4, Karlsruhe Institute of Technology, Karlsruhe, Germany
Institute of Organic Chemistry, Karlsruhe Institute of Technology, Karlsruhe, Germany
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Cited
17 citations as recorded by crossref.
- Real-Time Monitoring of Hydrolysis Reactions of Pyrophosphates with Dissolution Dynamic Nuclear Polarization J. Fukazawa et al. 10.1021/acs.jpclett.4c01456
- Short-lived calcium carbonate precursors observed in situ via Bullet-dynamic nuclear polarization E. Turhan et al. 10.1038/s42004-024-01300-5
- Fine optimization of a dissolution dynamic nuclear polarization experimental setting for 13C NMR of metabolic samples A. Dey et al. 10.5194/mr-3-183-2022
- Toward protein NMR at physiological concentrations by hyperpolarized water—Finding and mapping uncharted conformational spaces L. Epasto et al. 10.1126/sciadv.abq5179
- Post-acquisition correction of NMR spectra distorted by dynamic and static field inhomogeneity of cryogen-free magnets A. Karabanov et al. 10.1016/j.jmr.2023.107494
- Cryogen-free 400 MHz (9.4 T) solid state MAS NMR system with liquid state NMR potential E. Kryukov et al. 10.1016/j.ssnmr.2023.101873
- Optimization of heteronuclear ultrafast 2D NMR for the study of complex mixtures hyperpolarized by dynamic nuclear polarization C. Praud et al. 10.1039/D3AY01681A
- Primary Structure of Glycans by NMR Spectroscopy C. Fontana & G. Widmalm 10.1021/acs.chemrev.2c00580
- Radical-induced hetero-nuclear mixing and low-field 13C relaxation in solid pyruvic acid H. Kouřilová et al. 10.1039/D2CP04535D
- Bullet-DNP Enables NMR Spectroscopy of Pyruvate and Amino Acids at Nanomolar to Low Micromolar Concentrations P. Narwal et al. 10.1021/acs.analchem.4c00618
- Radical-Induced Low-Field 1H Relaxation in Solid Pyruvic Acid Doped with Trityl-OX063 M. Jurkutat et al. 10.1021/acs.jpclett.2c02357
- Introduction to “Geoffrey Bodenhausen Festschrift” D. Abergel & F. Ferrage 10.5194/mr-4-111-2023
- Spin Hyperpolarization in Modern Magnetic Resonance J. Eills et al. 10.1021/acs.chemrev.2c00534
- Hyperpolarization and sensitivity in nuclear magnetic resonance K. Kouřil & B. Meier 10.1016/j.jmro.2024.100172
- Long-lived enhanced magnetization—A practical metabolic MRI contrast material I. Katz et al. 10.1126/sciadv.ado2483
- Hyperpolarization of Long-Lived States of Protons in Aliphatic Chains by Bullet Dynamic Nuclear Polarization, Revealed on the Fly by Spin-Lock-Induced Crossing A. Razanahoera et al. 10.1021/acs.jpclett.4c01457
- Cryogenic sample eject system for electron paramagnetic resonance spectrometers K. Rieger et al. 10.1016/j.jmr.2024.107823
17 citations as recorded by crossref.
- Real-Time Monitoring of Hydrolysis Reactions of Pyrophosphates with Dissolution Dynamic Nuclear Polarization J. Fukazawa et al. 10.1021/acs.jpclett.4c01456
- Short-lived calcium carbonate precursors observed in situ via Bullet-dynamic nuclear polarization E. Turhan et al. 10.1038/s42004-024-01300-5
- Fine optimization of a dissolution dynamic nuclear polarization experimental setting for 13C NMR of metabolic samples A. Dey et al. 10.5194/mr-3-183-2022
- Toward protein NMR at physiological concentrations by hyperpolarized water—Finding and mapping uncharted conformational spaces L. Epasto et al. 10.1126/sciadv.abq5179
- Post-acquisition correction of NMR spectra distorted by dynamic and static field inhomogeneity of cryogen-free magnets A. Karabanov et al. 10.1016/j.jmr.2023.107494
- Cryogen-free 400 MHz (9.4 T) solid state MAS NMR system with liquid state NMR potential E. Kryukov et al. 10.1016/j.ssnmr.2023.101873
- Optimization of heteronuclear ultrafast 2D NMR for the study of complex mixtures hyperpolarized by dynamic nuclear polarization C. Praud et al. 10.1039/D3AY01681A
- Primary Structure of Glycans by NMR Spectroscopy C. Fontana & G. Widmalm 10.1021/acs.chemrev.2c00580
- Radical-induced hetero-nuclear mixing and low-field 13C relaxation in solid pyruvic acid H. Kouřilová et al. 10.1039/D2CP04535D
- Bullet-DNP Enables NMR Spectroscopy of Pyruvate and Amino Acids at Nanomolar to Low Micromolar Concentrations P. Narwal et al. 10.1021/acs.analchem.4c00618
- Radical-Induced Low-Field 1H Relaxation in Solid Pyruvic Acid Doped with Trityl-OX063 M. Jurkutat et al. 10.1021/acs.jpclett.2c02357
- Introduction to “Geoffrey Bodenhausen Festschrift” D. Abergel & F. Ferrage 10.5194/mr-4-111-2023
- Spin Hyperpolarization in Modern Magnetic Resonance J. Eills et al. 10.1021/acs.chemrev.2c00534
- Hyperpolarization and sensitivity in nuclear magnetic resonance K. Kouřil & B. Meier 10.1016/j.jmro.2024.100172
- Long-lived enhanced magnetization—A practical metabolic MRI contrast material I. Katz et al. 10.1126/sciadv.ado2483
- Hyperpolarization of Long-Lived States of Protons in Aliphatic Chains by Bullet Dynamic Nuclear Polarization, Revealed on the Fly by Spin-Lock-Induced Crossing A. Razanahoera et al. 10.1021/acs.jpclett.4c01457
- Cryogenic sample eject system for electron paramagnetic resonance spectrometers K. Rieger et al. 10.1016/j.jmr.2024.107823
Latest update: 04 Apr 2025
Short summary
The signals that are attainable in conventional NMR are tiny, and consequentially, NMR is often slow and insensitive. The intensity may be increased by hyperpolarization, provided that the spectral quality is essentially preserved. We have recently reported a potentially powerful hyperpolarization scheme, named bullet-DNP (dynamic nuclear polarization). Here, we report a 10-fold improvement in resolution for that method, which may enable new applications in metabolomics and drug discovery.
The signals that are attainable in conventional NMR are tiny, and consequentially, NMR is often...
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