Articles | Volume 2, issue 2
https://doi.org/10.5194/mr-2-815-2021
https://doi.org/10.5194/mr-2-815-2021
Research article
 | 
11 Nov 2021
Research article |  | 11 Nov 2021

A cryogen-free, semi-automated apparatus for bullet-dynamic nuclear polarization with improved resolution

Karel Kouřil, Michel Gramberg, Michael Jurkutat, Hana Kouřilová, and Benno Meier

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Cited articles

Ardenkjær-Larsen, J. H.: On the Present and Future of Dissolution-DNP, J. Magn. Reson., 264, 3–12, https://doi.org/10.1016/j.jmr.2016.01.015, 2016. a
Ardenkjær-Larsen, J. H.: Introduction to dissolution DNP: Overview, instrumentation, and human applications, Handbook of High Field Dynamic Nuclear Polarization, John Wiley & Sons, Chichester, UK, p. 239, 2019. a
Ardenkjær-Larsen, J. H., Fridlund, B., Gram, A., Hansson, G., Hansson, L., Lerche, M. H., Servin, R., Thaning, M., and Golman, K.: Increase in Signal-To-Noise Ratio of > 10 000 Times in Liquid-State NMR, P. Natl. Acad. Sci. USA, 100, 10158–10163, https://doi.org/10.1073/pnas.1733835100, 2003. a
Ardenkjaer-Larsen, J.-H., Boebinger, G. S., Comment, A., Duckett, S., Edison, A. S., Engelke, F., Griesinger, C., Griffin, R. G., Hilty, C., Maeda, H., Parigi, G., Prisner, T., Ravera, E., van Bentum, J., Vega, S., Webb, A., Luchinat, C., Schwalbe, H., and Frydman, L.: Facing and Overcoming Sensitivity Challenges in Biomolecular NMR Spectroscopy, Angew. Chem. Int. Edit., 54, 9162–9185, https://doi.org/10.1002/anie.201410653, 2015. a
Ardenkjær-Larsen, J. H., Bowen, S., Petersen, J. R., Rybalko, O., Vinding, M. S., Ullisch, M., and Nielsen, N. C.: Cryogen-Free Dissolution Dynamic Nuclear Polarization Polarizer Operating At 3.35 T, 6.70 T, and 10.1 T, Magn. Reson. Med., 81, 2184, https://doi.org/10.1002/mrm.27537, 2018. a
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Short summary
The signals that are attainable in conventional NMR are tiny, and consequentially, NMR is often slow and insensitive. The intensity may be increased by hyperpolarization, provided that the spectral quality is essentially preserved. We have recently reported a potentially powerful hyperpolarization scheme, named bullet-DNP (dynamic nuclear polarization). Here, we report a 10-fold improvement in resolution for that method, which may enable new applications in metabolomics and drug discovery.