Articles | Volume 3, issue 2
Research article
13 Sep 2022
Research article |  | 13 Sep 2022

Site-selective generation of lanthanoid binding sites on proteins using 4-fluoro-2,6-dicyanopyridine

Sreelakshmi Mekkattu Tharayil, Mithun C. Mahawaththa, Akiva Feintuch, Ansis Maleckis, Sven Ullrich, Richard Morewood, Michael J. Maxwell, Thomas Huber, Christoph Nitsche, Daniella Goldfarb, and Gottfried Otting


Interactive discussion

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on mr-2022-11', Daniel Häussinger, 18 Jul 2022
  • RC2: 'Comment on mr-2022-11', Nico Tjandra, 19 Jul 2022
  • EC1: 'Comment on mr-2022-11', Dominique Frueh, 21 Jul 2022
  • AC1: 'Comment on mr-2022-11', Gottfried Otting, 06 Aug 2022

Peer review completion

AR: Author's response | RR: Referee report | ED: Editor decision
AR by Gottfried Otting on behalf of the Authors (06 Aug 2022)  Author's response    Author's tracked changes    Manuscript
ED: Referee Nomination & Report Request started (09 Aug 2022) by Dominique Frueh
RR by Nico Tjandra (11 Aug 2022)
RR by Daniel Häussinger (11 Aug 2022)
ED: Publish as is (18 Aug 2022) by Dominique Frueh
AR by Gottfried Otting on behalf of the Authors (19 Aug 2022)  Author's response    Manuscript
Short summary
Having shown that tagging a protein at a single site with different lanthanoid complexes delivers outstanding structural information at a selected site of a protein (such as active sites and ligand binding sites), we now present a simple way by which different lanthanoid complexes can be assembled on a highly solvent-exposed cysteine residue. Furthermore, the chemical assembly is selective for selenocysteine, if a selenocysteine residue can be introduced into the protein of interest.