Site-selective generation of lanthanoid binding sites on proteins using 4-fluoro-2,6-dicyanopyridine
Sreelakshmi Mekkattu Tharayil,Mithun C. Mahawaththa,Akiva Feintuch,Ansis Maleckis,Sven Ullrich,Richard Morewood,Michael J. Maxwell,Thomas Huber,Christoph Nitsche,Daniella Goldfarb,and Gottfried Otting
Sreelakshmi Mekkattu Tharayil
Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Mithun C. Mahawaththa
ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Akiva Feintuch
Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel
Ansis Maleckis
Latvian Institute of Organic Synthesis, Aizkraukles 21, 1006 Riga, Latvia
Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Richard Morewood
Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Michael J. Maxwell
ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Thomas Huber
Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
Christoph Nitsche
Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia
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Having shown that tagging a protein at a single site with different lanthanoid complexes delivers outstanding structural information at a selected site of a protein (such as active sites and ligand binding sites), we now present a simple way by which different lanthanoid complexes can be assembled on a highly solvent-exposed cysteine residue. Furthermore, the chemical assembly is selective for selenocysteine, if a selenocysteine residue can be introduced into the protein of interest.
Having shown that tagging a protein at a single site with different lanthanoid complexes...
