Articles | Volume 4, issue 1
https://doi.org/10.5194/mr-4-1-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/mr-4-1-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
12 Jan 2023
Research article |
| 12 Jan 2023
| 12 Jan 2023
Intermolecular contributions, filtration effects and signal composition of SIFTER (single-frequency technique for refocusing)
Agathe Vanas
Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2,
8093 Zurich, Switzerland
Janne Soetbeer
Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2,
8093 Zurich, Switzerland
Frauke Diana Breitgoff
Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2,
8093 Zurich, Switzerland
Henrik Hintz
Department of Chemistry, Bielefeld University, Universitätsstrasse 25,
33615 Bielefeld, Germany
Muhammad Sajid
Department of Chemistry, Bielefeld University, Universitätsstrasse 25,
33615 Bielefeld, Germany
Yevhen Polyhach
Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2,
8093 Zurich, Switzerland
Adelheid Godt
Department of Chemistry, Bielefeld University, Universitätsstrasse 25,
33615 Bielefeld, Germany
Gunnar Jeschke
Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2,
8093 Zurich, Switzerland
Maxim Yulikov
CORRESPONDING AUTHOR
Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2,
8093 Zurich, Switzerland
Daniel Klose
CORRESPONDING AUTHOR
Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2,
8093 Zurich, Switzerland
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Dipolar electron paramagnetic resonance spectroscopy methods such as DEER provide data on how proteins change shape, thus giving detailed insight into how proteins work. We present DeerLab, a comprehensive open-source software for reliably analyzing the associated data. The software implements a series of theoretical and algorithmic innovations and thereby improves the quality and reproducibility of data analysis.
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Short summary
Nanometre distance measurements between spin labels by pulse EPR techniques yield structural information on the molecular level. Here, backed by experimental data, we derive a description for the total signal of the single-frequency technique for refocusing dipolar couplings (SIFTER), showing how the different spin–spin interactions give rise to dipolar signal and background – the latter has thus far been unknown.
Nanometre distance measurements between spin labels by pulse EPR techniques yield structural...
