Articles | Volume 4, issue 1
https://doi.org/10.5194/mr-4-27-2023
https://doi.org/10.5194/mr-4-27-2023
Research article
 | 
08 Feb 2023
Research article |  | 08 Feb 2023

The effect of the zero-field splitting in light-induced pulsed dipolar electron paramagnetic resonance (EPR) spectroscopy

Andreas Scherer, Berk Yildirim, and Malte Drescher

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Cited articles

Abdullin, D., Matsuoka, H., Yulikov, M., Fleck, N., Klein, C., Spicher, S., Hagelueken, G., Grimme, S., Luetzen, A., and Schiemann, O.: Pulsed EPR Dipolar Spectroscopy under the Breakdown of the High-Field Approximation: The High-Spin Iron(III) Case, Chem. Eur. J., 2, 8820–8828, https://doi.org/10.1002/chem.201900977, 2019. 
Bak, M. and Nielsen, N. C.: REPULSION, A Novel Approach to Efficient Powder Averaging in Solid-State NMR, J. Magn. Reson., 125, 132–139, https://doi.org/10.1006/jmre.1996.1087, 1997. 
Bertran, A., Henbest, K. B., De Zotti, M., Gobbo, M., Timmel, C. R., Di Valentin, M., and Bowen, A. M.: Light-Induced Triplet–Triplet Electron Resonance Spectroscopy, J. Phys. Chem. Lett., 12, 80–85, https://doi.org/10.1021/acs.jpclett.0c02884, 2020. 
Bertran, A., Barbon, A., Bowen, A. M., and Di Valentin, M.: Chapter Seven – Light-induced pulsed dipolar EPR spectroscopy for distance and orientation analysis, in: Methods in Enzymology, Vol. 666, edited by: Britt, R. D., Academic Press, 171–231, https://doi.org/10.1016/bs.mie.2022.02.012, 2022a. 
Bertran, A., Morbiato, L., Aquilia, S., Gabbatore, L., De Zotti, M., Timmel, C. R., Di Valentin, M., and Bowen, A. M.: Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy, Molecules, 27, 7526, https://doi.org/10.3390/molecules27217526, 2022b. 
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Short summary
Light-induced pulsed dipolar EPR spectroscopy (PDS) is an emerging field that uses photoexcited triplet states to determine distance restraints in the nanometer range. To date, light-induced PDS data have been analyzed with methods developed for techniques that do not invoke light-induced triplets. Here, we provide a new theoretical description that takes the full nature of the triplet state into account and demonstrate that it leads to more accurate fits of experimental data.