Pulsed electron paramagnetic resonance can measure distances and orientation between two paramagnetic markers. If they are rigidly attached to a biomolecule, advanced insights into the structure and dynamics of the biomolecule follow. We used chirp pulses to perform real two-dimensional experiments with much shorter experimental time compared to experiments with monochromatic microwave pulses. We also present new pulse sequences and give a detailed protocol for setting up such experiments.

In achiral polyfluoroalkyl substances with two to three CF₂ groups, long-lived ¹⁹F spin states (T_LLS) were measured and found to last about three times longer than T₁ in a static 11.6 T field. These lifetimes are sensitive to macromolecular binding, making them useful for screening fluorinated drugs.

The small protein GB1, where all valine residues were replaced by fluorinated analogues containing one or two CH₂F groups, produces ¹⁹F NMR (nuclear magnetic resonance) spectra with exceptional resolution. We establish a convenient strategy for their assignment and analyse the rotameric states of the CH₂F groups by virtue of three-bond coupling constants and a γ effect on ¹³C chemical shifts, which is underpinned by DFT (density functional theory) calculations. Transient fluorine–fluorine contacts are documented by through-space ¹⁹F–¹⁹F couplings.

By reviewing attendee lists of 10 MR (magnetic resonance) meetings over the last year, we estimate the climate footprint of conferences and explore possibilities to reduce it. This paper will facilitate discussions about possible actions the community may take.