Articles | Volume 2, issue 1
Magn. Reson., 2, 1–13, 2021

Special issue: Robert Kaptein Festschrift

Magn. Reson., 2, 1–13, 2021

Research article 06 Jan 2021

Research article | 06 Jan 2021

Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

Sreelakshmi Mekkattu Tharayil et al.

Data sets

NMR spectra Sreelakshmi Mekkattu Tharayil, Mithun Chamikara Mahawaththa, Ibidolapo Adekoya, and Gottfried Otting

Short summary
A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.