Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

Mekkattu Tharayil, Sreelakshmi; Mahawaththa, Mithun Chamikara; Loh, Choy-Theng; Adekoya, Ibidolapo; Otting, Gottfried

doi:https://doi.org/10.5194/mr-2-1-2021

Articles | Volume 2, issue 1

https://doi.org/10.5194/mr-2-1-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

Special issue:

Robert Kaptein Festschrift

https://doi.org/10.5194/mr-2-1-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 2, issue 1

Research article

|

06 Jan 2021

Research article |

| 06 Jan 2021

Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

Sreelakshmi Mekkattu Tharayil, Mithun Chamikara Mahawaththa, Choy-Theng Loh, Ibidolapo Adekoya, and Gottfried Otting

Supplement

https://doi.org/10.5194/mr-2-1-2021-supplement

Data sets

NMR spectra Sreelakshmi Mekkattu Tharayil, Mithun Chamikara Mahawaththa, Ibidolapo Adekoya, and Gottfried Otting https://doi.org/10.25911/5fc5bd5f0f872

Short summary

A new way is presented for creating lanthanide binding sites on proteins using site-specifically introduced phosphoserine residues. The paramagnetic effects of lanthanides generate long-range effects, which contain structural information and are readily measured by NMR spectroscopy. Excellent correlations between experimentally observed and back-calculated pseudocontact shifts attest to very good immobilization of the lanthanide ions relative to the proteins.